protein

Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor

MBS142098

0.02 mg

205 USD

Recombinant Protein

Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor

Granulocyte Macrophage-Colony Stimulating Factor

GM CSF Human; Granulocyte Macrophage-Colony Stimulating Factor Human Recombinant; CSF-2; MGI-1GM; GM-CSF; Pluripoietin-alpha; Molgramostin; Sargramostim; MGC131935; MGC138897; GM CSF

granulocyte-macrophage colony-stimulating factor; Granulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony-stimulating factor; CSF; granulocyte macrophage-colony stimulating factor; molgramostin; sargramostim; colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; Molgramostin; Sargramostim

GM

CSF2; CSF2; GMCSF; GMCSF; GM-CSF; CSF

CSF2_HUMAN

E Coli

144

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser.N-terminal methionine has been completely removed enzymatically.

Greater than 98.0% as determined by1. Analysis by RP-HPLC.2. Analysis by SDS-PAGE.

GM-CSF was lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH= 7.4 +/-0.1. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GMCSF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: GM-CSF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GEN computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard. Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.1 ng/ml, corresponding to a Specific Activity of 11,100,000 IU/mg.

CYTOKINES AND GROWTH FACTORS; Growth Factors; Colony Stimulating Factor

Description: Granulocyte Macrophage Colony Stimulating Factor Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton. GM-CSF is purified by proprietary chromatographic techniques. Introduction: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was first characterized as a growth factor that supports the in-vitro colony formation of granulocytes-macrophages progenitor cells (1, 2). It is a pleiotropic cytokine and a member of a family of endogenous cytokines of the hematopoietic system. GM-CSF is produced as a response to immune or inflammatory stimuli by activated cells of the hematopoietic system such as T cells, B cells, macrophages, mast cells and also fibroblasts and alveolar epithelial cells. It plays an important role in regulating the proliferation, differentiation, survival and activation of hematopoietic cells such as granulocytes and monocytes,neutrophiles, basophiles and eosonophoiles, erythroid cells, megakaryocytes and T cells (3,4). Human and mouse GM-CSF have about 56% homology and are species specific. Human GM-CSF is not active on mouse cells and vice versa. It is active on canine and feline cells (5, 6).GMCSF is 144 amino acids, 22kDa glycoprotein. It is composed of four bundles alpha helices. Its receptor is heterodimers with a ligand-specific alpha subunit and a betac (?c) subunit that is shared with the interleukin IL-3 and IL-5 receptors. This unusual form of receptor assembly likely applies also to IL-3 and IL-5 receptors. Cross-linking the two receptor subunits is required for receptor activation and signaling (7, 8).GMCSF has been shown to be involved in maturation, mobilization and antigen presentation of myeloid dentritic cells (DCs) in-vivo or ex-vivo. This function promotes Th1 immune responses, cytotoxcity, anti-angiogenesis as well as allergic inflammation, and the development of autoimmunity (9-11). Therefore GMCSF can be used in immunotherapy for the treatment of immune suppressed and immune-compromised patients as well as in veterinary medicine for the same purpose (12-14). GM-CSF is also important in regulation of embryo development and pregnancy and specifically in embryo implantation and subsequent development (15, 16).

27437030

NP_000749.2

NM_000758.3

P04141

16,295 Da

Amoebiasis Pathway 167324!!Amoebiasis Pathway 167191!!Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993!!Calcium Signaling In The CD4+ TCR Pathway 137941!!Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Cytokines And Inflammatory Response Pathway 198794!!Fc Epsilon RI Signaling Pathway 83082!!Fc Epsilon RI Signaling Pathway 493

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

0.02 mg

205 USD