Recombinant Human Granulocyte-Colony Stimulating Factor | MyBioSource MBS142097 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Granulocyte-Colony Stimulating Factor

catalog :

MBS142097

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142097

products type :

Recombinant Protein

products full name :

Recombinant Human Granulocyte-Colony Stimulating Factor

products short name :

Granulocyte-Colony Stimulating Factor

products name syn :

G CSF Human; Granulocyte-Colony Stimulating Factor Human Recombinant; CSF-3; MGI-1G; GM-CSF beta; Pluripoietin; Filgrastim; Lenograstim; G-CSF; MGC45931; GCSF

other names :

granulocyte colony-stimulating factor isoform a; Granulocyte colony-stimulating factor; granulocyte colony-stimulating factor; filgrastim; granulocyte-colony stimulating factor; lenograstim; pluripoietin; colony stimulating factor 3 (granulocyte); Pluripoietin; INN: Filgrastim; INN: Lenograstim

products gene name :

G CSF

other gene names :

CSF3; CSF3; GCSF; CSF3OS; C17orf33; C17orf33; GCSF; G-CSF

uniprot entry name :

CSF3_HUMAN

host :

E Coli

sequence length :

207

sequence :

The sequence of the first five N-terminal amino acids of GCSF was determined and was found to be Met-Thr-Pro-Leu-Gly.

purity :

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

form :

GCSF was lyophilized after extensive dialysis against 10mM sodium acetate buffer pH= 4. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Lyophilized GCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GCSF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

other info2 :

Solubility: It is recommended to reconstitute the lyophilized GCSF in sterile 20mM AcOH not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: GCSF quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GCSF as a Reference Standard. Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml, corresponding to a Specific Activity of 100,000,000 IU/mg.

products categories :

CYTOKINES AND GROWTH FACTORS; Growth Factors; Colony Stimulating Factor

products description :

Description: Granulocyte Colony Stimulating Factor Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18.8 KD.GCSF is purified by proprietary chromatographic techniques. Introduction: GCSF is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for the GCSF gene. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This csf induces granulocytes.

ncbi gi num :

4503079

ncbi acc num :

NP_000750.1

ncbi gb acc num :

NM_000759.3

uniprot acc num :

P09919

ncbi mol weight :

18,434 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Malaria Pathway (152665); Malaria Pathway (152657); PI3K-Akt Signaling Pathway (692234)

ncbi summary :

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

uniprot summary :

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell cycle regulation; Secreted, signal peptide; Cytokine; Secreted. Chromosomal Location of Human Ortholog: 17q11.2-q12. Cellular Component: extracellular space. Molecular Function: enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding. Biological Process: granulocyte differentiation; positive regulation of protein binding; positive regulation of myeloid cell differentiation; cytokine and chemokine mediated signaling pathway; multicellular organismal development; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; immune response

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

3490

more info or order :

MyBioSource product webpage