protein

Recombinant Human Keratinocyte Growth Factor

MBS142096

0.002 mg

140 USD

Recombinant Protein

Recombinant Human Keratinocyte Growth Factor

Keratinocyte Growth Factor

KGF Human; Keratinocyte Growth Factor Human Recombinant; HBGF-7; FGF7; FGF-7; KGF

fibroblast growth factor 7; Fibroblast growth factor 7; fibroblast growth factor 7; FGF-7; heparin-binding growth factor 7; keratinocyte growth factor; fibroblast growth factor 7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor

KGF

FGF7; FGF7; KGF; HBGF-7; KGF; FGF-7; HBGF-7

FGF7_HUMAN

E Coli

194

MCNDMTPEQM ATNVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNNYNIMEI RTVAVGIVAI KGVESEFYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHNG GEMFVALNQK GIPVRGKKTK KEQKTAHFLP MAIT.

Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Lyophilized from a 0.2?m filtered solution in 20mM PB, pH 8.0, 1M NaCl. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Keratinocyte Growth Factor1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution FGF7 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Keratinocyte Growth Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.9 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of KGF as a Reference Standard. Biological Activity: The biological activity was determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing KGF receptors yielding an ED50 <10ng/ml, corresponding to a Specific Activity of 1.0x105 IU/mg.

CYTOKINES AND GROWTH FACTORS; Growth Factors; Keratinocyte Growth Factor

Description: Keratinocyte Growth Factor-1 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 18995 Dalton.The FGF-7 is purified by proprietary chromatographic techniques. Introduction: KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.

4503705

NP_002000.1

NM_002009.3

P21781

11,520 Da

Activated Point Mutants Of FGFR2 Pathway (645281); Adaptive Immune System Pathway (366160); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signal Transduction Pathway (106385); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signaling Of Activated FGFR Pathway (160957); FGFR Ligand Binding And Activation Pathway (106344)

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq, Jul 2008]

FGF7: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. Belongs to the heparin-binding growth factors family. Protein type: Cytokine; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 15q21.2. Cellular Component: Golgi apparatus; extracellular region. Molecular Function: heparin binding; protein binding; growth factor activity; chemoattractant activity; fibroblast growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; epidermis development; fibroblast growth factor receptor signaling pathway; hair follicle morphogenesis; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; mesenchymal cell proliferation; signal transduction; positive regulation of peptidyl-tyrosine phosphorylation; positive chemotaxis; positive regulation of cell division; positive regulation of cell proliferation; actin cytoskeleton reorganization; response to wounding; insulin receptor signaling pathway; innate immune response; positive regulation of epithelial cell proliferation

0.002 mg

140 USD

0.01 mg

205

1 mg

4550