Recombinant Human Interleukin-11 | MyBioSource MBS142091 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Interleukin-11

catalog :

MBS142091

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142091

products type :

Recombinant Protein

products full name :

Recombinant Human Interleukin-11

products short name :

Interleukin-11

products name syn :

IL 11 Human; Interleukin-11 Human Recombinant; AGIF; Adipogenesis inhibitory factor; Oprelvekin; IL-11

other names :

interleukin-11 isoform 1; Interleukin-11; interleukin-11; adipogenesis inhibitory factor; oprelvekin; interleukin 11; Adipogenesis inhibitory factor

products gene name :

IL-11

other gene names :

IL11; IL11; AGIF; IL-11; IL-11

uniprot entry name :

IL11_HUMAN

host :

E Coli

sequence length :

199

sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Pro-Pro-Gly. N-terminal methionine has been completely removed enzymatically.

purity :

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

form :

The protein was lyophilized from a concentrated (1mg/ml) solution with no additives. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Lyophilized Interleukin-11 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL11 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

other info2 :

Solubility: It is recommended to reconstitute the lyophilized Interleukin 11 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.95 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IL-11 as a Reference Standard. Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of murine 7TD1 was found to be < 10ng/ml, corresponding to a Specific Activity of 100,000 IU/mg.

products categories :

CYTOKINES AND GROWTH FACTORS; Cytokines; Interleukin

products description :

Description: Interleukin-11 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 179 amino acids and having a molecular mass of 19256.29 Dalton. The IL-11 is purified by proprietary chromatographic techniques. Introduction: IL11 is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL6ST (gp130). IL-11 is shown to stimulate the T-cell-dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells.

ncbi gi num :

10834994

ncbi acc num :

NP_000632.1

ncbi gb acc num :

NM_000641.3

uniprot acc num :

P20809

ncbi mol weight :

12,994 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Integrated Pancreatic Cancer Pathway (711360); Interleukin-11 Signaling Pathway (698753); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Rheumatoid Arthritis Pathway (200309); Rheumatoid Arthritis Pathway (200269)

ncbi summary :

The protein encoded by this gene is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL6ST (gp130). This cytokine is shown to stimulate the T-cell-dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

uniprot summary :

IL11: Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Belongs to the IL-6 superfamily. Protein type: Secreted, signal peptide; Cell cycle regulation; Cytokine; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 19q13.3-q13.4. Cellular Component: extracellular space; cytoplasm; extracellular region. Molecular Function: growth factor activity; cytokine activity; interleukin-11 receptor binding. Biological Process: fat cell differentiation; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell signaling; positive regulation of MAPKKK cascade; megakaryocyte differentiation; negative regulation of hormone secretion; B cell differentiation; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

4550

more info or order :

MyBioSource product webpage