protein

Recombinant Human Interleukin-3

MBS142087

0.01 mg

205 USD

Recombinant Protein

Recombinant Human Interleukin-3

Interleukin-3

IL 3 Human; Interleukin-3 Human Recombinant; MCGF (Mast cell growth factor); Multi-CSF; HCGF; P-cell stimulation factor; IL-3; MGC79398; MGC79399

interleukin-3; Interleukin-3; interleukin-3; P-cell stimulating factor; P-cell-stimulating factor; colony-stimulating factor, multiple; hematopoietic growth factor; mast cell growth factor; mast-cell growth factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor; interleukin 3; Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor

IL 3

IL3; IL3; IL-3; MCGF; MULTI-CSF; IL-3; MCGF

IL3_HUMAN

E Coli

152

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Thr-Gln.

Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.

Lyophilized from a concentrated (1mg/ml) solution in water containing 0.3 mg/ml of NaHCO3. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL3 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.84 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard. Biological Activity: The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000 IU/mg.

CYTOKINES AND GROWTH FACTORS; Cytokines; Interleukin

Description: Interleukin-3 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The IL-3 is purified by proprietary chromatographic techniques. Introduction: IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

28416915

NP_000579.2

NM_000588.3

P08700

17,233 Da

Apoptosis Pathway 83060!!Apoptosis Pathway 470!!Asthma Pathway 83120!!Asthma Pathway 532!!Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993!!Calcium Signaling In The CD4+ TCR Pathway 137941!!Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Fc Epsilon RI Signaling Pathway 83082

The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]

0.01 mg

205 USD