protein

Recombinant Human Interleukin-2

MBS142086

0.01 mg

140 USD

Recombinant Protein

Recombinant Human Interleukin-2

Interleukin-2

IL 2 Human; Interleukin-2 Human Recombinant; T-cell growth factor (TCGF); Aldesleukin; Lymphokine; IL-2

interleukin-2; Interleukin-2; interleukin-2; T cell growth factor; aldesleukin; involved in regulation of T-cell clonal expansion; interleukin 2; T-cell growth factor; TCGF; INN: Aldesleukin

IL-2

IL2; IL2; IL-2; TCGF; lymphokine; IL-2; TCGF

IL2_HUMAN

E Coli

153

MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFCQSII STLT.

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

The protein (1mg/ml) was lyophilized after extensive dialysis against 20mM PBS pH=7.4. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL2 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Interleukin 2 in sterile distilled water or 20mM AcOH not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000 IU/mg.

CYTOKINES AND GROWTH FACTORS; Cytokines; Interleukin

Description: Interleukin-2 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15kDa. The IL-2 is purified by proprietary chromatographic techniques. Introduction: IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

28178861

NP_000577.2

NM_000586.3

P60568

17,628 Da

AhR Pathway (755436); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Calcium Signaling In The CD4+ TCR Pathway (137941); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Corticotropin-releasing Hormone Pathway (920957)

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]

IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family. Protein type: Secreted; Secreted, signal peptide; Oncoprotein; Cytokine. Chromosomal Location of Human Ortholog: 4q26-q27. Cellular Component: extracellular space; extracellular region. Molecular Function: growth factor activity; interleukin-2 receptor binding; cytokine activity; kinase activator activity; carbohydrate binding; glycosphingolipid binding; kappa-type opioid receptor binding. Biological Process: positive regulation of isotype switching to IgG isotypes; negative regulation of heart contraction; natural killer cell activation; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; elevation of cytosolic calcium ion concentration; negative regulation of protein amino acid phosphorylation; cell-cell signaling; positive regulation of cell proliferation; protein kinase C activation; positive regulation of B cell proliferation; cell adhesion; T cell differentiation; positive regulation of interleukin-17 production; regulation of T cell homeostatic proliferation; positive regulation of regulatory T cell differentiation; positive regulation of immunoglobulin secretion; positive regulation of cell growth; positive regulation of interferon-gamma production; positive regulation of tissue remodeling; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of inflammatory response; immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of B cell apoptosis; positive regulation of inflammatory response; negative regulation of apoptosis

0.01 mg

140 USD

0.05 mg

205

1 mg

765