Recombinant Human Brain-Derived Neurotrophic Factor | MyBioSource MBS142084 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Brain-Derived Neurotrophic Factor

catalog :

MBS142084

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142084

products type :

Recombinant Protein

products full name :

Recombinant Human Brain-Derived Neurotrophic Factor

products short name :

Brain-Derived Neurotrophic Factor

products name syn :

BDNF Human; Brain-Derived Neurotrophic Factor Human Recombinant; Brain-Derived Neurotrophic Factor; BDNF; MGC34632

other names :

brain-derived neurotrophic factor isoform a preproprotein; Brain-derived neurotrophic factor; brain-derived neurotrophic factor; abrineurin; neurotrophin; brain-derived neurotrophic factor; Abrineurin

products gene name :

BDNF

other gene names :

BDNF; BDNF; ANON2; BULN2; BDNF

uniprot entry name :

BDNF_HUMAN

host :

E Coli

sequence length :

247

sequence :

The sequence of the first five N-terminal amino acids of BDNF was determined and found to be Met-His-Ser-Asp-Pro.

purity :

BDNF is greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

form :

The protein was lyophilized WITHOUT ANY ADDITIVES. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Lyophilized BDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution BDNF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

other info2 :

Solubility: It is recommended to reconstitute the lyophilized BDNF in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: BDNF quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Brain-derived Neurotrophic Factor as a Reference Standard. Biological Activity: The ED50, calculated by the dose-dependent induction of ACHE (acetylcholine esterase) in rat basal forebrain primary septal culture is 50 ng/ml corresponding to a Specific Activity of 20,000IU/mg.

products categories :

CYTOKINES AND GROWTH FACTORS; Neurotrophins

products description :

Description: Brain-Derived Neurotrophic Factor Human Recombinant produced in E Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids and having a total molecular mass of 26,984 Dalton. BDNF Human Recombinant is purified by proprietary chromatographic techniques. Introduction: BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. BDNF is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the cns. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (ltp), long-term depression (ltd), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

ncbi gi num :

219842288

ncbi acc num :

NP_001137277.1

ncbi gb acc num :

NM_001143805.1

uniprot acc num :

P23560

ncbi mol weight :

31,116 Da

ncbi pathways :

Alcoholism Pathway (585563); Alcoholism Pathway (587116); BDNF Signaling Pathway (712093); Cocaine Addiction Pathway (546258); Cocaine Addiction Pathway (546273); FSH Signaling Pathway (672455); Huntington's Disease Pathway (83100); Huntington's Disease Pathway (512); Integrated Pancreatic Cancer Pathway (711360); MAPK Signaling Pathway (198779)

ncbi summary :

The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009]

uniprot summary :

BDNF: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. Belongs to the NGF-beta family. 5 isoforms of the human protein are produced by alternative promoter. Protein type: Cell development/differentiation; Cytokine; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 11p13. Cellular Component: extracellular space; mitochondrial crista; synaptic vesicle; perinuclear region of cytoplasm; cytoplasmic membrane-bound vesicle; dendrite; cytoplasm; extracellular region; terminal button; perikaryon. Molecular Function: growth factor activity; neurotrophin TRKB receptor binding. Biological Process: circadian rhythm; axon guidance; mechanoreceptor differentiation; behavioral fear response; mitochondrial electron transport, NADH to ubiquinone; axon extension; regulation of neuron differentiation; response to hormone stimulus; positive regulation of long-term neuronal synaptic plasticity; response to vitamin A; negative regulation of neuroblast proliferation; ureteric bud development; dendrite development; regulation of retinal cell programmed cell death; feeding behavior; negative regulation of neuron apoptosis; response to electrical stimulus; negative regulation of synaptic transmission, GABAergic; inner ear development; response to food; nervous system development; chronic inflammatory response; response to light intensity; neuron recognition; learning; regulation of short-term neuronal synaptic plasticity; regulation of axon extension; negative regulation of striated muscle development; positive regulation of peptidyl-serine phosphorylation; positive regulation of synaptogenesis; response to hyperoxia; axon target recognition; glutamate secretion; response to hypoxia; response to fluoxetine; nerve development; response to activity; positive regulation of neuron differentiation; regulation of excitatory postsynaptic membrane potential; gamma-aminobutyric acid signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; neurite morphogenesis. Disease: Obsessive-compulsive Disorder; Bulimia Nervosa, Susceptibility To, 1; Bulimia Nervosa, Susceptibility To, 2; Central Hypoventilation Syndrome, Congenital

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

4550

more info or order :

MyBioSource product webpage