protein

Recombinant Human Erythropoietin-alpha

MBS142078

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Erythropoietin-alpha

Erythropoietin-alpha

EPO a Human; Erythropoietin-Alpha Human Recombinant; Erythropoietin-Alpha; EPO-a; EPO-alpha; Epoetin; EP; MGC138142; EPO a

erythropoietin; Erythropoietin; erythropoietin; epoetin; erythropoietin; INN: Epoetin

EPO

EPO; EPO; EP; MVCD2

EPO_HUMAN

Chinese Hamster Ovarian Cells (CHO)

193

APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR.

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Each mg of lyophilized powder contains 0.59 mg sodium citrate, 0.58 mg sodium chloride and 0.006 mg citric acid. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution EPO-alpha should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Epoetin-a in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The Specific Activity was measured by Normocyth -aemic mice and was found to be 150,000 IU/mg.

CYTOKINES AND GROWTH FACTORS; Growth Factors; Erythropoietin

Description: Erythropoietin-alpha Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 166 amino acids and having a predicted molecular mass of 21,000 Dalton and apparent glycosylated molecular mass of 36-40kDa. EPO-a is purified by proprietary chromatographic techniques. Introduction: This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

62240997

NP_000790.2

NM_000799.2

P01588

21,307 Da

Cellular Response To Hypoxia Pathway (645259); Cellular Responses To Stress Pathway (645258); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); EPO Receptor Signaling Pathway (198882); EPO Signaling Pathway (138051); HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); HIF-2-alpha Transcription Factor Network Pathway (137956); Hematopoietic Cell Lineage Pathway (83078)

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008]

EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 7q22. Cellular Component: extracellular space; cell surface; extracellular region. Molecular Function: protein binding; erythropoietin receptor binding; hormone activity; protein kinase activator activity. Biological Process: hemoglobin biosynthetic process; erythrocyte maturation; apoptosis; blood circulation; positive regulation of transcription, DNA-dependent; response to lipopolysaccharide; response to testosterone stimulus; signal transduction; response to salt stress; response to vitamin A; peptidyl-serine phosphorylation; response to hyperoxia; positive regulation of tyrosine phosphorylation of Stat5 protein; response to estrogen stimulus; positive regulation of protein kinase activity; positive regulation of cell proliferation; response to axon injury; positive regulation of Ras protein signal transduction; positive regulation of neuron differentiation; response to electrical stimulus; positive regulation of DNA replication; embryo implantation; aging. Disease: Microvascular Complications Of Diabetes, Susceptibility To, 2

0.005 mg

140 USD

0.05 mg

205

1 mg

1365