protein

Recombinant Mouse GRO/KC (CXCL1)

MBS142042

0.005 mg

140 USD

Recombinant Protein

Recombinant Mouse GRO/KC (CXCL1)

GRO/KC

GRO1/KC Mouse; GRO1/KC Mouse Recombinant (CXCL1); Growth-regulated alpha protein; CXCL1; Platelet-derived growth factor-inducible protein KC; Secretory protein N51; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1; chemokine (C-X-C motif) ligand 1

growth-regulated alpha protein; Growth-regulated alpha protein; growth-regulated alpha protein; C-X-C motif chemokine 1; GRO1 oncogene; KC/GR)-alpha; KC/GRO-alpha; alpha-chemokine; platelet-derived growth factor-inducible protein KC; secretory protein N51; chemokine (C-X-C motif) ligand 1; C-X-C motif chemokine 1; Platelet-derived growth factor-inducible protein KC; Secretory protein N51KC(5-72)Alternative name(s):Hematopoietic synergistic factor; HSF; KC-T

mGRO1/KC

Cxcl1; Cxcl1; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1; Gro; Gro1; Mgsa; Scyb1; HSF

GROA_MOUSE

E Coli

96

RLATGAPIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK.

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

The protein was lyophilized with no additives. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized KC Mouse although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CXCL1 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized GRO1 Recombinant in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.03 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of KC as a Reference Standard. Biological Activity: The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with ?50ng/mL of recombinant mouse KC (Specific Activity: ? 2 x 104 units/mg).

CHEMOKINES; Chemokines; GRO

Description: KC Mouse Recombinant also known as N51 and GRO1 produced in E Coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of approximately 8 kDa.The GRO-1 is purified by proprietary chromatographic techniques. Introduction: Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-a). It is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines.

6680109

NP_032202.1

NM_008176.3

P12850

10,254 Da

Chemokine Receptors Bind Chemokines Pathway (1110501); Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (1110499); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response (BioCarta) Pathway (198283); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1111486); Disease Pathway (1111319)

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung. [provided by RefSeq, Apr 2013]

CXCL2: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted. Cellular Component: extracellular space; cell; extracellular region; cytosol. Molecular Function: growth factor activity; chemokine activity; CXCR chemokine receptor binding; cytokine activity. Biological Process: G-protein coupled receptor protein signaling pathway; neutrophil chemotaxis; elevation of cytosolic calcium ion concentration; positive regulation of superoxide release; response to molecule of bacterial origin; positive regulation of potassium ion transport; immune response; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; inflammatory response; acute inflammatory response; regulation of cell proliferation

0.005 mg

140 USD

0.02 mg

205

1 mg

2665