protein

Recombinant Human Exodus-2 (CCL21)

MBS142039

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Exodus-2 (CCL21)

Exodus-2

Exodus 2 Human; Exodus-2 Human Recombinant (CCL21); Small inducible cytokine A21; CCL21; Beta chemokine exodus-2; 6Ckine; Secondary lymphoid-tissue chemokine; SLC; chemokine (C-C motif) ligand 21; ECL; CKb9; TCA4; SCYA21; MGC34555; Exodus 2

C-C motif chemokine 21; C-C motif chemokine 21; C-C motif chemokine 21; Efficient Chemoattractant for Lymphocytes; beta chemokine exodus-2; secondary lymphoid tissue chemokine; small inducible cytokine subfamily A (Cys-Cys), member 21; chemokine (C-C motif) ligand 21; 6Ckine; Beta-chemokine exodus-2; Secondary lymphoid-tissue chemokine; SLC; Small-inducible cytokine A21

CCL21; CCL21; ECL; SLC; CKb9; TCA4; 6Ckine; SCYA21; SCYA21; SLC

CCL21_HUMAN

E Coli

134

SDGGAQDCCL KYSQRKIPAK VVRSYRKQEP SLGCSIPAIL FLPRKRSQAE LCADPKELWV QQLMQHLDKT PSPQKPAQGC RKDRGASKTG KKGKGSKGCR KTERSQTPKG P.

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Lyophilized from a 0.2 um filtered concentrated solution in 20mM Phosphate Buffer, pH 7.4 and 150mM NaCl. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Exodus-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CCL21 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Exodus2 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: Determined by its ability to chemoattract human total lymphocyte population using a concentration range of 10-100ng/ml.

CHEMOKINES; Chemokines

Description: Exodus-2 Human Recombinant produced in E Coli as a single, non-glycosylated, polypeptide chain containing 111 amino acids and having a molecular mass of 12219 Dalton. The CCL21 is purified by proprietary chromatographic techniques. Introduction: Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). CCL21 is expressed predominantly in the lymph nodes and, in contrast to other CC chemokines, is chemotactic for lymphocytes. The gene for CCL21 is located on human chromosome 9. CCL21 elicits its effects by binding to a cell surface chemokine receptor known as CCR7.

4506835

NP_002980.1

NM_002989.3

O00585

14,646 Da

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548)

This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19). [provided by RefSeq, Sep 2014]

CCL21: Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Belongs to the intercrine beta (chemokine CC) family. Protein type: Secreted; Chemokine; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9p13. Cellular Component: extracellular space; extracellular region. Molecular Function: chemokine activity; chemokine receptor binding; CCR7 chemokine receptor binding. Biological Process: positive regulation of filopodium formation; positive regulation of I-kappaB kinase/NF-kappaB cascade; formation of immunological synapse; positive regulation of chemotaxis; positive regulation of dendritic cell antigen processing and presentation; cell maturation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of cell adhesion mediated by integrin; positive regulation of JNK cascade; positive regulation of receptor-mediated endocytosis; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; positive regulation of pseudopodium formation; cell-cell signaling; positive regulation of actin filament polymerization; positive regulation of protein kinase activity; release of sequestered calcium ion into cytosol; T cell costimulation; ruffle organization and biogenesis; dendritic cell chemotaxis; immune response; establishment of T cell polarity; inflammatory response; positive regulation of cell-matrix adhesion

0.005 mg

140 USD

0.02 mg

205

1 mg

2665