protein

Recombinant Human Epithelial Neutrophil-Activating Protein 78 (CXCL5)

MBS142038

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Epithelial Neutrophil-Activating Protein 78 (CXCL5)

Epithelial Neutrophil-Activating Protein 78

ENA 78 Human; Epithelial Neutrophil-Activating Protein 78 Human Recombinant (CXCL5); Small inducible cytokine B5; CXCL5; Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; ENA-78(1-78); chemokine (C-X-C motif) ligand 5; SCYB5

C-X-C motif chemokine 5; C-X-C motif chemokine 5; C-X-C motif chemokine 5; epithelial-derived neutrophil-activating protein 78; neutrophil-activating peptide ENA-78; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78); chemokine (C-X-C motif) ligand 5; ENA-78(1-78); Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; Small-inducible cytokine B5Cleaved into the following 2 chains:ENA-78(8-78); ENA-78(9-78)

ENA 78

CXCL5; CXCL5; SCYB5; ENA-78; ENA78; SCYB5

CXCL5_HUMAN

E Coli

114

The sequence of the first five N-terminal amino acids was determined and was found to be, Ala- Ala -Val-Leu-Arg.

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

The CXCL5 was lyophilized from a concentrated (1mg/ml) solution in water containing no additives. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized ENA78 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CXCL5 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized ENA-78 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with 100ng/mL (corresponding to a Specific Activity of 10,000IU/mg) of recombinant human ENA-78.

CHEMOKINES; Chemokines; ENA 78

Description: Epithelial Neutrophil-Activating Protein 78 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8020 Dalton. The CXCL5 is purified by proprietary chromatographic techniques. Introduction: Chemokine (C-X-C motif) ligand 5 (CXCL5) is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-?. This chemokine stimulates the chemotaxis of neutrophils possesses angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on human chromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodelling.

4506849

NP_002985.1

NM_002994.4

P42830

11,972 Da

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548)

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils, to promote angiogenesis and to remodel connective tissues. This protein is thought to play a role in cancer cell proliferation, migration, and invasion. [provided by RefSeq, May 2013]

CXCL5: Involved in neutrophil activation. In vitro, ENA-78(8- 78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q13.3. Cellular Component: extracellular space; extracellular region. Molecular Function: chemokine activity; CXCR chemokine receptor binding. Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; positive regulation of cell proliferation; neutrophil mediated immunity; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; immune response; signal transduction; inflammatory response; chemotaxis

0.005 mg

140 USD

0.02 mg

205

1 mg

2665