protein

Recombinant Human Eotaxin (CCL11)

MBS142010

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Eotaxin (CCL11)

Eotaxin

Eotaxin Human; Eotaxin Human Recombinant (CCL11); Small inducible cytokine A11; CCL11; Eosinophil chemotactic protein; chemokine (C-C motif) ligand 11; SCYA11; MGC22554; Eotaxin

eotaxin; Eotaxin; eotaxin; eosinophil chemotactic protein; eotaxin-1; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin); chemokine (C-C motif) ligand 11; C-C motif chemokine 11; Eosinophil chemotactic protein; Small-inducible cytokine A11

CCL11; CCL11; SCYA11; SCYA11

CCL11_HUMAN

E Coli

97

The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Ala-Ser-Val.

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Lyophilized from a 0.2 um filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Eotaxin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CCL11 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Patent Protected Countries: The Sale of Eotaxin Human Recombinant by MyBioSource is prohibited in the following countries: United States, Germany, Australia, Austria, Belgium, Switzerland (including Liechentstein), Denmark, Spain, France, Greece, Ireland, Italy, Luxembourg, Monaco, The Netherlands, Portugal, Sweden and The United Kingdom. Solubility: It is recommended to reconstitute the lyophilized Eotaxin Human Recombinant in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.013 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Eotaxin as a Reference Standard. Biological Activity: The activity is determined by the chemoattract of human PBE (peripheral blood eosinophils) at a concentration between 0.1-10 ng/ml corresponding to a Specific Activity of 100,000-10,000,000IU/mg.

CHEMOKINES; Chemokines; Eotaxin

Description: Eotaxin Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 74 amino acids and having a molecular mass of 8345.9 Dalton. The CCL11 is purified by proprietary chromatographic techniques. Introduction: Chemokine (C-C motif) ligand 11 (CCL11) is a small cytokine belonging to the CC chemokine family that is also known as eotaxin. CCL11 selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses. The effects of CCL11 are mediated by its binding to a G-protein-linked receptor known as a chemokine receptor. Chemokine receptors for which CCL11 is a ligand include CCR2, CCR3 and CCR5. The gene for human CCL11 (scya11) is encoded on three exons and is located on chromosome 17.

4506827

NP_002977.1

NM_002986.2

P51671

10,732 Da

Asthma Pathway (83120); Asthma Pathway (532); CXCR3-mediated Signaling Events Pathway (138011); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664)

This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil-specific chemokine is thought to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. [provided by RefSeq, Sep 2014]

CCL11: In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3. Belongs to the intercrine beta (chemokine CC) family. Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted. Chromosomal Location of Human Ortholog: 17q12. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; chemokine activity. Biological Process: chronic inflammatory response; mast cell chemotaxis; response to virus; actin filament organization; cytoskeleton organization and biogenesis; signal transduction; chemotaxis; protein amino acid phosphorylation; cellular calcium ion homeostasis; regulation of cell shape; response to radiation; positive regulation of angiogenesis; positive regulation of actin filament polymerization; eosinophil chemotaxis; positive regulation of endothelial cell proliferation; immune response; inflammatory response; cell adhesion; positive regulation of cell migration. Disease: Asthma, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To

0.005 mg

140 USD

0.02 mg

205

1 mg

2665