Recombinant Human Macrophage Inflammatory protein-1 alpha (CCL3) | MyBioSource MBS142009 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Macrophage Inflammatory protein-1 alpha (CCL3)

catalog :

MBS142009

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142009

products type :

Recombinant Protein

products full name :

Recombinant Human Macrophage Inflammatory protein-1 alpha (CCL3)

products short name :

Macrophage Inflammatory protein-1 alpha

products name syn :

MIP 1a Human; Macrophage Inflammatory Protein-1 Alpha Human Recombinant (CCL3); Small inducible cytokine A3; CCL3; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; Tonsillar lymphocyte LD78 alpha protein; G0/G1 switch regulatory protein 19-1; G0S19-1 protein; SIS-beta; PAT 464.1; chemokine (C-C motif) ligand 3; MIP1A; SCYA3; G0S19-1; LD78ALPHA

other names :

C-C motif chemokine 3; C-C motif chemokine 3; C-C motif chemokine 3; G0/G1 switch regulatory protein 19-1; PAT 464.1; SIS-beta; macrophage inflammatory protein 1-alpha; small inducible cytokine A3 (homologous to mouse Mip-1a); tonsillar lymphocyte LD78 alpha protein; chemokine (C-C motif) ligand 3; G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; PAT 464.1; SIS-beta; Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha proteinMIP-1-alpha(4-69)Alternative name(s):LD78-alpha(4-69)

products gene name :

MIP 1a

other gene names :

CCL3; CCL3; MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha; G0S19-1; MIP1A; SCYA3; MIP-1-alpha

uniprot entry name :

CCL3_HUMAN

host :

E Coli

sequence length :

sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Ser-Leu-Ala-Ala.

purity :

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

form :

The protein was lyophilized from 0.55 mg/ml solution containing no additives. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Lyophilized MIP-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CCL3 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

other info2 :

Solubility: It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-1a in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The Activity is calculated by the ability of chemo-attraction of Human monocytes using 1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.

products categories :

CHEMOKINES; Chemokines; MIP

products description :

Description: Macrophage Inflammatory Protein-1 alpha Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7820 Dalton. The MIP-1a is purified by proprietary chromatographic techniques. Introduction: Macrophage Inflammatory Proteins (MIP) belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1a and MIP-1b that are now officially named CCL3 and CCL4 respectively. Both are major factors produced by macrophages after they are stimulated with bacterial endotoxins. They activate human granulocytes (neutrophils, eosinophilsand basophils) which can lead to acute neutrophilic inflammation. They also induce the synthesis and release of other pro-inflammatory cytokines such as interleukin 1 (IL-1), IL-6 and TNF-a from fibroblasts and macrophages. The genes for CCL3 and CCL4 are both located on human chromosome 17.

ncbi gi num :

4506843

ncbi acc num :

NP_002974.1

ncbi gb acc num :

NM_002983.2

uniprot acc num :

P10147

ncbi mol weight :

10,085 Da

ncbi pathways :

Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764)

ncbi summary :

This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]

uniprot summary :

CCL3: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Belongs to the intercrine beta (chemokine CC) family. Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Chemokine. Chromosomal Location of Human Ortholog: 17q12. Cellular Component: extracellular space; cytoplasm; extracellular region; intracellular; cytosol. Molecular Function: identical protein binding; protein binding; CCR1 chemokine receptor binding; chemokine activity; calcium-dependent protein kinase C activity; kinase activity; chemoattractant activity; CCR5 chemokine receptor binding; protein kinase activity; phospholipase activator activity. Biological Process: positive regulation of catalytic activity; negative regulation of osteoclast differentiation; exocytosis; behavior; response to toxin; positive regulation of calcium-mediated signaling; positive regulation of interleukin-1 beta secretion; chemotaxis; protein amino acid phosphorylation; monocyte chemotaxis; regulation of cell shape; negative regulation of bone mineralization; positive chemotaxis; cell-cell signaling; positive regulation of neuron apoptosis; calcium ion transport; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; inflammatory response; lymphocyte chemotaxis; neutrophil chemotaxis; MAPKKK cascade; calcium-mediated signaling; cytoskeleton organization and biogenesis; positive regulation of tumor necrosis factor production; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; macrophage chemotaxis; cellular calcium ion homeostasis; osteoblast differentiation; cell activation; positive regulation of protein kinase B signaling cascade; eosinophil chemotaxis; eosinophil degranulation; regulation of sensory perception of pain; positive regulation of calcium ion transport; astrocyte cell migration; positive regulation of inflammatory response; positive regulation of cell migration. Disease: Human Immunodeficiency Virus Type 1, Susceptibility To

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

2665

more info or order :

MyBioSource product webpage