protein

Recombinant Human cyclin-dependent kinase 1

MBS141153

0.005 mg

140 USD

Recombinant Protein

Recombinant Human cyclin-dependent kinase 1

[cyclin-dependent kinase 1]

[CDK1 Human; Cyclin-Dependent Kinase 1 Human Recombinant; Cyclin-Dependent Kinase 1; CDC2; Cell Division Cycle 2; G1 To S And G2 To M; Cell Division Control Protein 2 Homolog; Cell Division Protein Kinase 1; P34 Protein Kinase; P34CDC2; CDC28A; Cell Cycle Controller CDC2; EC 2.7.11.22; EC 2.7.11.23; CDKN1; Cyclin-dependent kinase 1; CDK1]

[cyclin-dependent kinase 1 isoform 1; Cyclin-dependent kinase 1; cyclin-dependent kinase 1; cyclin-dependent kinase 1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase]

[CDK1]

[CDK1; CDK1; CDC2; CDC28A; P34CDC2; CDC2; CDC28A; CDKN1; P34CDC2; CDK1]

CDK1_HUMAN

Escherichia Coli

[1-297]

297

MGSSHHHHHH SSGLVPRGSH MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR EISLLKELRH PNIVSLQDVLMQDSRLYLIF EFLSMDLKKY LDSIPPGQYM DSSLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDDKGTIKLADFGLA RAFGIPIRVY THEVVTLWYR SPEVLLGSAR YSTPVDIWSI GTIFAELATK KPLFHGDSEIDQLFRIFRAL GTPNNEVWPE VESLQDYKNT FPKWKPGSLA SHVKNLDENG LDLLSKMLIY DPAKRISGKMALNHPYFNDL DNQIKKM

Greater than 85.0% as determined by SDS-PAGE.

CDK1 protein solution (1mg/ml) containing 20mM Tris-HCl (pH8.0) and 10% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Physical Appearance: Sterile filtered colorless solution.

PROTEIN KINASES

Introduction: Cyclin-dependent kinase 1 (CDK1) plays a significant part in the control of the eukaryotic cell cycle through modulating the centrosome cycle in addition to mitotic onset; CDK1 promotes G2-M transition and regulates G1 progress and G1-S transition using association with multiple interphase cyclins. CDK1 is essential in higher cells for the entry into S-phase and mitosis. Description: CDK1 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 317 amino acids (1-297 a.a) and having a molecular mass of 36.2kDa. CDK1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

4502709

NP_001777.1

NM_001786.4

P06493

27,503 Da

APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (1269838); APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway (1269848); APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (1269844); APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway (1269845); Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (1269842); Activation Of NIMA Kinases NEK9, NEK6, NEK7 Pathway (1269816); Anchoring Of The Basal Body To The Plasma Membrane Pathway (1268847); Assembly Of The Primary Cilium Pathway (1268846); Cdc20:Phospho-APC/C Mediated Degradation Of Cyclin A Pathway (1269846); Cell Cycle Pathway (1269741)

CDK1: a protein kinase of the CDK family. Catalytic subunit of the conserved protein complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions. Mitotic cyclins stably associate with this protein and function as regulatory subunits. Its activity is controlled by cyclin availability and phosphorylation through the cell cycle. Activated in many cancers including colon, liver and breast. The T isoform, which lacks a regulatory region, is expressed in breast cancer. Inhibition in cancer cells may drive cells into apoptosis. May also drive cell migration. Inhibitors: BMS-265246, BMS-265246-01. 2 isoforms of the human protein are produced by alternative splicing. Protein type: CDK family; CDK/CDK1 subfamily; CDK1 subfamily; CMGC group; Cell cycle regulation; EC 2.7.11.22; EC 2.7.11.23; Kinase, protein; Motility/polarity/chemotaxis; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor). Chromosomal Location of Human Ortholog: 10q21.1. Cellular Component: centrosome; cytoplasm; cytosol; membrane; midbody; mitochondrion; nuclear chromosome, telomeric region; nucleoplasm; nucleus; spindle microtubule. Molecular Function: cyclin binding; cyclin-dependent protein kinase activity; histone kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity; RNA polymerase subunit kinase activity. Biological Process: activation of MAPK activity; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell migration; centrosome cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA repair; DNA replication; epithelial cell differentiation; G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; G2/M transition of mitotic cell cycle; microtubule cytoskeleton organization and biogenesis; mitotic nuclear envelope disassembly; negative regulation of apoptosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; pronuclear fusion; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of embryonic development; regulation of Schwann cell differentiation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle

0.005 mg

140 USD

0.02 mg

205

1 mg

2665