Mouse Anti Human Axin-1 | MyBioSource MBS140367 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti Human Axin-1

catalog :

MBS140367

quantity :

0.005 mg

price :

140 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PAT1A4AT

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS140367

products type :

Antibody

products full name :

Mouse Anti Human Axin-1

products short name :

Axin-1

products name syn :

Axin1 Antibody; Axin-1, Mouse Anti Human; Axin-1; Axis inhibition protein 1; hAxin; AXIN1; AXIN; MGC52315

other names :

axin-1 isoform a; Axin-1; axin-1; axis inhibition protein 1; axis inhibitor 1; fused, mouse, homolog of; protein phosphatase 1, regulatory subunit 49; axin 1; Axis inhibition protein 1; hAxin

products gene name :

AXIN1

other gene names :

AXIN1; AXIN1; AXIN; PPP1R49; AXIN; hAxin

uniprot entry name :

AXIN1_HUMAN

clonality :

Monoclonal

isotype :

IgG2b heavy chain and kappa light chain

clone :

PAT1A4AT

host :

Mouse

sequence length :

862

purity :

Axin1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

form :

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

storage stability :

For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles. 12 months at -20 degree C. 1 month at 4 degree C.

tested application :

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

app notes :

Axin1 antibody has been tested by ELISA, Western blot, Immunofluorescence, and Immunoprecipitation analyses to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:500.

other info2 :

Immunogen: Anti-human Axin1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Axin1 amino acids 546-752 purified from E Coli.

products categories :

MONOCLONAL/POLYCLONAL ANTIBODIES, MISCELLANEOUS ANTIBODIES; Monoclonal Antibodies

products description :

Introduction: AXIN1 is a cytoplasmic protein, which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. Axin1 is has been characterized as a tumor suppressor. In addition, Axin1 has an essential role in the formation of the embryonic neural axis. It has also been reported that Axin is found in mitotic spindles and centrosomes. AXIN1 interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. AXIN1 functions as a negative regulator of the WNT1 signaling pathway and is able to induce apoptosis. Axin can be identified in a mouse mutant strain, created by random integration of a transgene. Mutations in the AXIN1 gene are linked to hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas.

ncbi gi num :

27501450

ncbi acc num :

NP_003493.1

ncbi gb acc num :

NM_003502.3

ncbi mol weight :

91,653 Da

ncbi pathways :

AMER1 Mutants Destabilize The Destruction Complex Pathway (1127621); APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway (1127616); APC Truncation Mutants Have Impaired AXIN Binding Pathway (1127617); AXIN Missense Mutants Destabilize The Destruction Complex Pathway (1127620); AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway (1127618); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Beta-catenin Phosphorylation Cascade Pathway (106512); C-MYC Pathway (169344); Canonical Wnt Signaling Pathway (138032)

ncbi summary :

This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2010]

uniprot summary :

axin 1: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin-1 functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Wild-type axin 1 can induce apoptosis in hepatocellular and colorectal cancer cells. Is downregulated during progression of esophageal squamous cell carcinoma. Mutation of the axin-1 gene is associated with hepatocellular carcinoma, anaplastic thyroid cancer, medulloblastoma and colorectal cancer. May have a role in oncogenesis in Hodgkin lymphoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways. Protein type: Tumor suppressor; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; cytoplasmic microtubule; cytoplasmic membrane-bound vesicle; postsynaptic density; cytoplasm; cell cortex; cytoplasmic vesicle; nucleus; beta-catenin destruction complex; cytosol; lateral plasma membrane. Molecular Function: protein C-terminus binding; identical protein binding; protein homodimerization activity; protein self-association; p53 binding; beta-catenin binding; protein kinase binding; GTPase activator activity; protein binding; signal transducer activity; enzyme binding; receptor signaling complex scaffold activity; ubiquitin protein ligase binding; protein complex scaffold; SMAD binding; receptor binding. Biological Process: cell death; negative regulation of Wnt receptor signaling pathway; protein polyubiquitination; apoptosis; embryonic eye morphogenesis; positive regulation of transcription, DNA-dependent; positive regulation of JNK cascade; dorsal/ventral axis specification; nucleocytoplasmic transport; negative regulation of protein metabolic process; Wnt receptor signaling pathway through beta-catenin; olfactory placode formation; cytoplasmic microtubule organization and biogenesis; activation of JNK activity; sensory perception of sound; positive regulation of ubiquitin-protein ligase activity; Wnt receptor signaling pathway involved in forebrain neuron fate commitment; protein catabolic process; protein homooligomerization; axial mesoderm formation; in utero embryonic development; positive regulation of transforming growth factor beta receptor signaling pathway; muscle cell development; negative regulation of fat cell differentiation; optic placode formation; positive regulation of peptidyl-serine phosphorylation; cellular protein complex assembly; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; forebrain anterior/posterior pattern formation; positive regulation of protein catabolic process; activation of protein kinase activity; positive regulation of protein amino acid phosphorylation; determination of left/right symmetry; positive regulation of GTPase activity. Disease: Caudal Duplication Anomaly; Hepatocellular Carcinoma

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

0.1 mg

price3 :

505

more info or order :

MyBioSource product webpage