Mouse Anti Human Glyceraldehyde-3-Phosphate Dehydrogenase | MyBioSource MBS140362 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti Human Glyceraldehyde-3-Phosphate Dehydrogenase

catalog :

MBS140362

quantity :

0.02 mg

price :

205 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PAT8G4AT

reactivity :

rat

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS140362

products type :

Antibody

products full name :

Mouse Anti Human Glyceraldehyde-3-Phosphate Dehydrogenase

products short name :

Glyceraldehyde-3-Phosphate Dehydrogenase

products name syn :

GAPDH Antibody; Glyceraldehyde-3-Phosphate Dehydrogenase, Mouse Anti Human; G3PD; GAPD; MGC88685; GAPDH; Glyceraldehyde-3-Phosphate Dehydrogenase

other names :

glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; 38 kDa BFA-dependent ADP-ribosylation substrate; peptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde-3-phosphate dehydrogenase; 38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)

products gene name :

GAPDH

other gene names :

Gapdh; Gapdh; Gapd; BARS-38; Gapd; GAPDH

uniprot entry name :

G3P_RAT

clonality :

Monoclonal

isotype :

IgG2b heavy chain and kappa light chain

clone :

PAT8G4AT

host :

Mouse

sequence length :

333

purity :

GAPDH antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

form :

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. Sterile Filtered clear solution.

storage stability :

For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles. 12 months at -20 degree C. 1 month at 4 degree C.

tested application :

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)

app notes :

GAPDH antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:500 ~ 10,000. Recommended starting dilution is 1:1,000.

other info2 :

Immunogen: Anti-human GAPDH mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human GAPDH amino acids 1-335 purified from E Coli.

products categories :

MONOCLONAL ANTIBODIES, ANTI-HUMAN ENZYMES; Monoclonal Antibodies; Anti Human Enzyme

products description :

Introduction: GAPDH is a catalytic enzyme normally known to play a role in glycolysis. GAPDH exists as a tetramer composed of 36-kDa subunits and has a range of intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, GAPDH has function in intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. GAPDH catalyzes a vital energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

ncbi gi num :

8393418

ncbi acc num :

NP_058704.1

ncbi gb acc num :

NM_017008.4

ncbi mol weight :

35,828 Da

ncbi pathways :

Alzheimer's Disease Pathway 83489!!Alzheimer's Disease Pathway 509!!Androgen Receptor Signaling Pathway 198529!!Biosynthesis Of Amino Acids Pathway 791250!!Biosynthesis Of Amino Acids Pathway 795174!!Carbon Metabolism Pathway 816129!!Carbon Metabolism Pathway 817567!!Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway 434661!!Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway 468196!!Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate 434659

ncbi summary :

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. A similar protein in human and mouse has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies in human and mouse have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins. Many pseudogenes similar to this locus are found throughout the rat genome. [provided by RefSeq, Jan 2014]

size :

0.02 mg

price :

205 USD

more info or order :

MyBioSource product webpage