catalog number :
MBS140269
products full name :
Mouse Anti Human Homeobox Protein NANOG
products short name :
Homeobox Protein NANOG
products name syn :
NANOG Antibody; Homeobox Protein NANOG, Mouse Anti Human; NANOG; Homeobox protein NANOG; Homeobox transcription factor Nanog; hNanog
other names :
homeobox protein NANOG isoform 1; Homeobox protein NANOG; homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Homeobox transcription factor Nanog; hNanog
products gene name :
NANOG
other gene names :
NANOG; NANOG; hNanog
uniprot entry name :
NANOG_HUMAN
isotype :
IgG2a heavy chain and kappa light chain
purity :
NANOG antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
form :
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
storage stability :
For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles. 12 months at -20 degree C. 1 month at 4 degree C.
tested application :
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
app notes :
NANOG antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500~1:1,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:500 and Immunohistochemistry is 1:50.
other info2 :
Immunogen: Anti-human NANOG mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human NANOG amino acids 1-154 purified from E Coli.
products categories :
MONOCLONAL/POLYCLONAL ANTIBODIES, MISCELLANEOUS ANTIBODIES; Monoclonal Antibodies
products description :
Introduction: NANOG is a multidomain homeobox transcription factor which functions to maintain the undifferentiated state of pluripotent stem cells. NANOG expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is downregulated, cell differentiation can proceed. Proteins which regulate NANOG expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
ncbi acc num :
NP_079141.2
ncbi gb acc num :
NM_024865.3
ncbi mol weight :
32,838 Da
ncbi pathways :
Cardiac Progenitor Differentiation Pathway 712094!!Developmental Biology Pathway 477129!!POU5F1 (OCT4), SOX2, NANOG Activate Genes Related To Proliferation Pathway 1127697!!POU5F1 (OCT4), SOX2, NANOG Repress Genes Related To Differentiation Pathway 1127698!!Proteoglycans In Cancer Pathway 782000!!Proteoglycans In Cancer Pathway 782054!!SIDS Susceptibility Pathways 198901!!Signaling Pathways Regulating Pluripotency Of Stem Cells 1026136!!Signaling Pathways Regulating Pluripotency Of Stem Cells 1033502!!Transcriptional Regulation Of Pluripotent Stem Cells Pathway 1127696