catalog number :
MBS140232
products full name :
Mouse Anti Human CD73
products short name :
CD73
products name syn :
CD73 Antibody; CD73, Mouse Anti Human; 5'-nucleotidase; Ecto-5'-nucleotidase; 5'-NT; CD73; NT5E; NT5; NTE; NT; eN; eNT; E5NT
products gene name :
CD73
isotype :
IgG2b heavy chain and kappa light chain
purity :
CD73 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
form :
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. Sterile filtered colorless solution.
storage stability :
For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles. 12 months at -20 degree C. 1 month at 4 degree C.
tested application :
ELISA (EIA), Western Blot (WB)
app notes :
CD73 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.
other info2 :
Immunogen: Anti-human CD73 mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CD73 amino acids 27-252 purified from E Coli.
products categories :
MONOCLONAL ANTIBODIES, ANTI-HUMAN LYMPHOCYTES; Monoclonal Antibodies; Anti Human Lymphocyte
products description :
Introduction: Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP at a neutral pH. CD73, which consists of a dimer of 2 identical 70kDa subunits, is bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. CD73 is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Additional forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.