antibody

Mouse Anti Human CASP8 and FADD-like apoptosis regulator

MBS140222

0.005 mg

140 USD

monoclonal

mouse

nonconjugated

P5D8AT

western blot, ELISA, enzyme immunoassay

Antibody

Mouse Anti Human CASP8 and FADD-like apoptosis regulator

CASP8 and FADD-like apoptosis regulator

CFLAR Antibody; CASP8 and FADD-like apoptosis regulator, Mouse Anti Human; CASP8 and FADD-like apoptosis regulator; Cellular FLICE-like inhibitory protein; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; MACH-related inducer of toxicity; Caspase homolog; Inhibitor of FLICE; FADD-like antiapoptotic molecule 1; Usurpin; c-FLIP; Casper; CLARP; MRIT; CASH; I-FLICE; FLAME-1; CFLAR; CASP8AP1

CASP8 and FADD-like apoptosis regulator isoform 6; CASP8 and FADD-like apoptosis regulator; CASP8 and FADD-like apoptosis regulator; FADD-like antiapoptotic molecule 1; MACH-related inducer of toxicity; caspase homolog; caspase-eight-related protein; caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; cellular FLICE-like inhibitory protein; inhibitor of FLICE; usurpin beta; CASP8 and FADD-like apoptosis regulator; Caspase homolog; CASH; Caspase-eight-related protein; Casper; Caspase-like apoptosis regulatory protein; CLARP; Cellular FLICE-like inhibitory protein; c-FLIP; FADD-like antiapoptotic molecule 1; FLAME-1; Inhibitor of FLICE; I-FLICE; MACH-related inducer of toxicity; MRIT; UsurpinCleaved into the following 2 chains:CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12

CFLAR

CFLAR; CFLAR; CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1; CASH; CASP8AP1; CLARP; MRIT; CASH; Casper; CLARP; c-FLIP; FLAME-1; I-FLICE; MRIT

CFLAR_HUMAN

Monoclonal

IgG3 heavy chain and kappa light chain

P5D8AT

Mouse

366

CFLAR antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles. 12 months at -20 degree C. 1 month at 4 degree C.

ELISA (EIA), Western Blot (WB)

CFLAR antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.

Immunogen: Anti-human CFLAR mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CFLAR amino acids 1-376 purified from E Coli.

MONOCLONAL/POLYCLONAL ANTIBODIES, MISCELLANEOUS ANTIBODIES; Monoclonal Antibodies

Introduction: CFLAR contains two death effector domains (DEDs) and a caspase-like domain. CFLAR may play a critical role between cell survival and cell death pathway in mammalian cells. CFLAR also interacts with adapter protein FADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors DR3 (death receptor 3), TRAIL-R (TNF-related apoptosis-inducing ligand receptor) and TNFR1 (tumor necrosis factor receptor 1).

321267573

NP_001189448.1

NM_001202519.1

44,112 Da

Apoptosis Pathway (198797); Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Pathway (105648); Apoptosis Modulation And Signaling Pathway (198822); Caspase-8 Activation By Cleavage Pathway (105654); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Death Receptor Signalling Pathway (105650); Dimerization Of Procaspase-8 Pathway (105655)

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]

CFLAR: Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. TNFRSF6 stimulation triggers recruitment to the death- inducing signaling complex (DISC) formed by TNFRSF6, FADD and caspase-8. A proteolytic fragment (p43) stays associated with the DISC. Also interacts with caspase-10, caspase-3, TRAF1, TRAF2 and Bcl-X(L) (in vitro). Interacts with HBV protein X. Repressed by IL2/interleukin-2 after TCR stimulation, during progression to the S phase of the cell cycle. Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. Belongs to the peptidase C14A family. 14 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis. Chromosomal Location of Human Ortholog: 2q33-q34. Cellular Component: cytoplasm; CD95 death-inducing signaling complex; cytosol; lipid raft. Molecular Function: protein binding; protease binding; cysteine-type endopeptidase activity; enzyme activator activity; death receptor binding; protein complex binding. Biological Process: caspase activation; skeletal muscle development; skeletal muscle atrophy; positive regulation of I-kappaB kinase/NF-kappaB cascade; skeletal myofibril assembly; viral reproduction; apoptosis; skeletal muscle regeneration; regulation of satellite cell proliferation; proteolysis; activation of NF-kappaB transcription factor; negative regulation of apoptosis

0.005 mg

140 USD

0.02 mg

205

0.1 mg

505