antibody

Mouse Anti Human BH3 Interacting Domain Death Agonist

MBS140216

0.02 mg

205 USD

monoclonal

mouse

nonconjugated

P4D3AT

western blot, ELISA, enzyme immunoassay

Antibody

Mouse Anti Human BH3 Interacting Domain Death Agonist

BH3 Interacting Domain Death Agonist

BID Antibody; BH3 Interacting Domain Death Agonist, Mouse Anti Human; BH3-interacting domain death agonist; p22 BID; BID; FP497; MGC15319; MGC42355

BH3-interacting domain death agonist isoform 2; BH3-interacting domain death agonist; BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; Human BID coding sequence; apoptic death agonist; desmocollin type 4; p22 BID; BH3 interacting domain death agonist; p22 BID; BID

BID

BID; BID; FP497; BID

BID_HUMAN

Monoclonal

IgG1 heavy chain and kappa light chain

P4D3AT

Mouse

195

BID antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles. 12 months at -20 degree C. 1 month at 4 degree C.

ELISA (EIA), Western Blot (WB)

BID antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.

Immunogen: Anti-human BID mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human BID amino acids 1-195 purified from E Coli.

MONOCLONAL/POLYCLONAL ANTIBODIES, MISCELLANEOUS ANTIBODIES; Monoclonal Antibodies

Introduction: BID accession number NP_001187 is a pro-apoptotic Bcl-2 protein having only the BH3 domain. In reaction to apoptotic signaling, BID interacts with another Bcl-2 family of cell death regulators, called Bax, they form a heterodimer resulting to the insertion of Bax into the outer mitochondrial membrane. Bax induces the opening of the mitochondrial voltage-dependent anion channel which leads to the release of cytochrome c and other pro-apoptotic factors from the mitochondria resulting in activation of caspases. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8). CASP8 cleaves BID, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. The major proteolytic product p15 BID releasea cytochrome c. Isoform 1, Isoform 2 and Isoform 4 induce ice-like proteases and apoptosis while Isoform 3 does not induce apoptosis.

4557361

NP_001187.1

NM_001196.3

11,263 Da

Activation And Oligomerization Of BAK Protein Pathway 105666!!Activation Of BAD And Translocation To Mitochondria Pathway 105659!!Activation Of BH3-only Proteins Pathway 105658!!Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway 105657!!Activation, Translocation And Oligomerization Of BAX Pathway 105665!!Alzheimer's Disease Pathway 83097!!Alzheimer's Disease Pathway 509!!Alzheimers Disease Pathway 672448!!Amyotrophic Lateral Sclerosis (ALS) Pathway 920975!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83099

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

0.02 mg

205 USD