Mouse Anti Human Geminin | MyBioSource MBS140178 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti Human Geminin

catalog :

MBS140178

quantity :

0.005 mg

price :

140 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P2H7AT

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS140178

products type :

Antibody

products full name :

Mouse Anti Human Geminin

products short name :

Geminin

products name syn :

GMNN Antibody; Geminin, Mouse Anti Human; GMNN; Geminin; DNA Replication Inhibitor; Gem; RP3-369A17.3

other names :

geminin; Geminin; geminin; geminin, DNA replication inhibitor

products gene name :

GMNN

other gene names :

GMNN; GMNN; Gem

uniprot entry name :

GEMI_HUMAN

clonality :

Monoclonal

isotype :

IgG1 heavy chain and kappa light chain

clone :

P2H7AT

host :

Mouse

sequence length :

209

purity :

Geminin antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

form :

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

storage stability :

For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles. 12 months at -20 degree C. 1 month at 4 degree C.

tested application :

ELISA (EIA), Western Blot (WB)

app notes :

Geminin antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.

other info2 :

Immunogen: Anti-human Geminin mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Geminin amino acids 1-209 purified from E Coli.

products categories :

MONOCLONAL/POLYCLONAL ANTIBODIES, MISCELLANEOUS ANTIBODIES; Monoclonal Antibodies

products description :

Introduction: Geminin is a 25 kDa nuclear protein, which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. Geminin controls replication by binding to the licensing factor Cdt1, and is involved in neural differentiation. In addition, Geminin directly interacts with Six3 and Hox homeodomain proteins during embryogenesis and inhibits their functions. Geminin can also promote DNA replication. Geminin has 2 roles in 2 different stages of the cell cycle: Geminin is a negative regulator of DNA replication during the œS phase of the cell cycle. Inhibition of Geminin during the œS phase (by RNAi) results in an additional round of replication of portions of the genome. During the œM phase of the cell cycle (mitosis) Geminin stabilizes the replication factor Cdt1 promoting DNA replication during the next cell cycle. Moreover, inhibition of Geminin during mitosis (by RNAi) causes destabilization of Cdt1 protein and impairment of DNA replication during the next cell cycle. G

ncbi gi num :

354681980

ncbi acc num :

NP_001238918.1

ncbi gb acc num :

NM_001251989.1

ncbi mol weight :

23,565 Da

ncbi pathways :

Assembly Of The Pre-replicative Complex Pathway (105821); Association Of Licensing Factors With The Pre-replicative Complex Pathway (105900); CDT1 Association With The CDC6:ORC:origin Complex Pathway (105824); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); DNA Replication Pathway (198771); DNA Replication Pathway (105897); DNA Replication Pre-Initiation Pathway (105898); M/G1 Transition Pathway (105820); Regulation Of DNA Replication Pathway (105899)

ncbi summary :

This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011]

uniprot summary :

Geminin: Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Homotetramer. Interacts with CDT1; inhibits binding of the MCM complex to origins of replication. Interacts with IDAS; targets GMNN to the nucleus, prevents GMNN interaction with CDT1 and competes with IDAS homodimerization. Belongs to the geminin family. Protein type: Cell cycle regulation; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 6p22.3. Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol. Molecular Function: protein binding; histone deacetylase binding; transcription corepressor activity. Biological Process: organ morphogenesis; negative regulation of DNA replication; protein complex assembly; mitotic cell cycle; negative regulation of transcription, DNA-dependent; negative regulation of cell cycle

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

0.1 mg

price3 :

505

more info or order :

MyBioSource product webpage