Mouse Anti Human Clusterin | MyBioSource MBS140166 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti Human Clusterin

catalog :

MBS140166

quantity :

0.005 mg

price :

140 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P1A11AT

reactivity :

rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS140166

products type :

Antibody

products full name :

Mouse Anti Human Clusterin

products short name :

Clusterin

products name syn :

Clusterin Antibody; Clusterin, Mouse Anti Human; CLI; AAG4; KUB1; SGP2; SGP-2; SP-40; TRPM2; MGC24903; Complement-associated protein SP-40; 40; Complement cytolysis inhibitor; NA1/NA2; Apolipoprotein J; Apo-J; Testosterone-repressed prostate message 2; TRPM-2; Clusterin

other names :

clusterin; Clusterin; clusterin; dimeric acid glycoprotein; sulfated glycoprotein 2; testosterone repressed prostate message 2; testosterone-repressed prostate message; testostrone-repressed prostate message 2; clusterin; Dimeric acid glycoprotein; DAG; Sulfated glycoprotein 2; SGP-2; Testosterone repressed prostate message 2; TRPM-2

other gene names :

Clu; Clu; CLI; DAG; APOJ; SGP2; SP40; SGP-2; SP-40; Trpm2; Trpmb; TRPM-2; TRPM2B; RATTRPM2B; DAG; SGP-2; TRPM-2

uniprot entry name :

CLUS_RAT

clonality :

Monoclonal

isotype :

IgG1 heavy chain and kappa light chain

clone :

P1A11AT

host :

Mouse

sequence length :

447

purity :

Clusterin antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

form :

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. Sterile Filtered clear solution.

storage stability :

For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles. 12 months at -20 degree C. 1 month at 4 degree C.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Clusterin antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:100~200. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:100.

other info2 :

Immunogen: Anti-human Clusterin mAb, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with Recombinant human Clusterin amino acids 1-333 purified from E Coli.

products categories :

ANTI-HUMAN CYTOKINES; Monoclonal Antibodies; Anti Human Cytokine

products description :

Introduction: Clusterin also named Apolipoprotein J (APO-J) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the ? and ? chains. These are assembled in anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil ? -helices and three predicted amphipathic a-helices. Across a broad range of species clusterin shows a high degree of sequence homology ranging from 70% to 80%. It is nearly ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, b

ncbi gi num :

46048420

ncbi acc num :

NP_444180.2

ncbi gb acc num :

NM_053021.2

ncbi mol weight :

51,375 Da

ncbi pathways :

Hemostasis Pathway (1120520); Platelet Activation, Signaling And Aggregation Pathway (1120530); Platelet Degranulation Pathway (1120547); Response To Elevated Platelet Cytosolic Ca2+ Pathway (1120545)

ncbi summary :

The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders.[provided by RefSeq, May 2011]

uniprot summary :

CLU: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. Belongs to the clusterin family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Mitochondrial; Apoptosis; Secreted; Secreted, signal peptide. Cellular Component: extracellular matrix; extracellular space; neuron projection; growth cone; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; mitochondrial membrane; cytoplasm; cytosol; nucleus. Molecular Function: protein binding; ubiquitin protein ligase binding; ATPase activity; protein N-terminus binding; misfolded protein binding. Biological Process: response to light stimulus; response to misfolded protein; positive regulation of nitric oxide biosynthetic process; protein stabilization; positive regulation of apoptosis; response to virus; cell morphogenesis; microglial cell activation; positive regulation of tumor necrosis factor production; endocrine pancreas development; negative regulation of protein homooligomerization; activation of NF-kappaB transcription factor; myelin maintenance in the central nervous system; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein import; positive regulation of cell proliferation; response to wounding; spermatogenesis; response to oxidative stress; positive regulation of cell differentiation; chaperone-mediated protein complex assembly; neurite morphogenesis; aging; negative regulation of apoptosis

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

0.1 mg

price3 :

505

more info or order :

MyBioSource product webpage