Mouse Anti Human Vimentin Biotinylated | MyBioSource MBS140124 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti Human Vimentin Biotinylated

catalog :

MBS140124

quantity :

0.5 mg

price :

410 USD

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

hVimentin

reactivity :

human, mouse, rat

application :

immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS140124

products type :

Antibody

products full name :

Mouse Anti Human Vimentin Biotinylated

products short name :

Vimentin Biotinylated

products name syn :

Vimentin Antibody, Biotin; Vimentin, Mouse Anti Human Antibody, Biotin; Vimentin Biotin

other names :

vimentin; Vimentin; vimentin; epididymis luminal protein 113; vimentin

other gene names :

VIM; VIM; HEL113; CTRCT30

uniprot entry name :

VIME_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

hVimentin

host :

Mouse

reactivity :

Vimentin antibody will recognize most mammalian Vimentin except mouse and rat.

sequence length :

466

purity :

Ion exchange column.

concentration :

1mg/ml in PBS (after reconstitution).

storage stability :

In lyophilized form, for long periods, store at 4 degree C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20 degree C. Antibody is shipped lyophilized at ambient temperature.

tested application :

Immunohistochemistry (IHC)

app notes :

For intra-cellular staining, use 5-10 ul/1,000,000 cells. Wash twice with PBS/Azide before adding the appropriate amount of secondary fluorescent reagent.

other info1 :

Other Available Forms: This antibody is also available conjugated to FITC and non conjugate form.

other info2 :

Reconstitution: Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Immunogen: Purified Human Vimentin.

products categories :

MONOCLONAL/POLYCLONAL ANTIBODIES, MISCELLANEOUS ANTIBODIES; Monoclonal Antibodies

products description :

Introduction: Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines.

ncbi gi num :

62414289

ncbi acc num :

NP_003371.2

ncbi gb acc num :

NM_003380.3

ncbi mol weight :

53,652 Da

ncbi pathways :

Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Apoptotic Cleavage Of Cellular Proteins Pathway (105678); Apoptotic Execution Phase Pathway (105677); Aurora B Signaling Pathway (138080); Caspase Cascade In Apoptosis Pathway (137974); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (105679); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); MicroRNAs In Cancer Pathway (852705)

ncbi summary :

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

uniprot summary :

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: 10p13. Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol. Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens. Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; muscle filament sliding; cell structure disassembly during apoptosis. Disease: Cataract 30

size1 :

0.5 mg

price1 :

410 USD

size2 :

1 mg

price2 :

550

more info or order :

MyBioSource product webpage