catalog number :
MBS140109
products full name :
Mouse Anti FLAG peptide Peroxidase Conjugated
products short name :
FLAG peptide Peroxidase Conjugated
products name syn :
FLAG-HRP Antibody; FLAG Peptide, Mouse Antibody Peroxidase Conjugated; FLAG peptide HRP
products gene name :
FLAG
uniprot entry name :
C1DCR6_LARHH
form :
1xPBS & 50% glycerol.
tested application :
Western Blot (WB)
app notes :
Western Blotting: 1:4000.
products categories :
MONOCLONAL/POLYCLONAL ANTIBODIES, MISCELLANEOUS ANTIBODIES; Monoclonal Antibodies
products description :
Description: Monoclonal antibodies are produced by immunizing mouse with synthetic peptide (DYKDDDDK), coupled to KLH. Introduction: A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody denoted as M1 binds the peptide in the presence of bivalent metal cations preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide.The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins.
ncbi acc num :
YP_002796694.1
ncbi gb acc num :
NC_012559.1
ncbi mol weight :
15,150 Da