antibody

Mouse Anti Human FAS (CD95) Blocking

MBS140095

0.5 mg

230 USD

monoclonal

mouse

nonconjugated

NYRhFAS

ELISA, enzyme immunoassay

Antibody

Mouse Anti Human FAS (CD95) Blocking

FAS

FAS Antibody; FAS Blocking Antibody (CD95), Mouse anti Human; FASLG receptor; Apoptosis-mediating surface antigen FAS; Apo-1 antigen; CD95; Tumor necrosis factor receptor superfamily member 6 TNR6; APT1; FAS1; TNFRSF6

fatty acid synthase; Fatty acid synthase; fatty acid synthase; short chain dehydrogenase/reductase family 27X, member 1; fatty acid synthase

FAS

FASN; FASN; FAS; OA-519; SDR27X1; FAS

FAS_HUMAN

Monoclonal

IgG1

NYRhFAS

Mouse

2511

Ion exchange column Protein concentration1 mg/ml in PBS (after reconstitution).

In lyophilized form, for long periods, store at 4 degree C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20 degree C. Antibody is shipped lyophilized at ambient temperature.

ELISA (EIA)

By direct ELISA, 1:10,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig.

Additional Information: This is a BLOCKING antibody and will block FAS-mediated apoptosis.

Reconstitution: Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Immunogen: Recombinant Human FAS.

ANTI-HUMAN CYTOKINES; Monoclonal Antibodies; Anti Human Cytokine

Introduction: The Fas receptor (CD95) mediates apoptotic signaling by Fas-ligand expressed on the surface of other cells. The Fas-FasL interaction plays an important role in the immune system and lack of this system leads to autoimmunity, indicating that Fas-mediated apoptosis removes self-reactive lymphocytes. Fas signaling is also involved in immune surveillance to remove transformed cells and virus infected cells. Binding of FAS to oligimerized FasL on another cell activates apoptotic signaling through a cytoplasmic domain termed the death domain that interacts with signaling adaptors including FAF, FADD and DAX to activate the caspase proteolytic cascade. Caspase-8 and caspase-10 are first activated, to then cleave and activate downstream caspases, and a variety of cellular substrates that lead to cell death. Caspases cleave nuclear lamins, causing the nucleus to break down and lose its normal structure and another caspase substrate is DFF, inducing cleavage and degradation of the genome. Other caspase substrates are involved in cytoskeletal structure, cell cycle regulation and signaling pathways. Activation of JNK kinase, activation of Jun, and production of ceramide may also play roles in Fas-mediated apoptosis. Activation of fas-mediated apoptosis is opposed by I-FLICE and FAP. Viruses and tumors may escape immune surveillance in part through suppression of fas-mediated apoptosis using similar mechanisms.

41872631

NP_004095.4

NM_004104.4

273,427 Da

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Activation Of Gene Expression By SREBF (SREBP) Pathway (685552); ChREBP Activates Metabolic Gene Expression Pathway (106104); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004)

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]

FASN: a multifunctional enzyme. Catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. Protein type: EC 2.3.1.85; EC 4.2.1.59; Acetyltransferase; EC 1.1.1.100; EC 2.3.1.38; EC 2.3.1.39; EC 2.3.1.41; EC 3.1.2.14; Lyase; EC 1.3.1.39; Oxidoreductase; Hydrolase; Lipid Metabolism - fatty acid biosynthesis. Chromosomal Location of Human Ortholog: 17q25. Cellular Component: Golgi apparatus; glycogen granule; membrane; mitochondrion; cytoplasm; plasma membrane; melanosome; cytosol. Molecular Function: protein homodimerization activity; fatty-acid synthase activity; myristoyl-[acyl-carrier-protein] hydrolase activity; 3-oxoacyl-[acyl-carrier-protein] synthase activity; zinc ion binding; enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity; enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity; drug binding; [acyl-carrier-protein] S-acetyltransferase activity; 3-oxoacyl-[acyl-carrier-protein] reductase activity; protein binding; 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; oleoyl-[acyl-carrier-protein] hydrolase activity; [acyl-carrier-protein] S-malonyltransferase activity; palmitoyl-[acyl-carrier-protein] hydrolase activity; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity. Biological Process: osteoblast differentiation; vitamin metabolic process; acetyl-CoA metabolic process; pantothenate metabolic process; positive regulation of cellular metabolic process; energy reserve metabolic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; fatty acid metabolic process; fatty acid biosynthetic process; water-soluble vitamin metabolic process

0.5 mg

230 USD

1 mg

350