antibody

Mouse Anti Maltose-Binding protein

MBS140073

0.02 mg

325 USD

monoclonal

mouse

nonconjugated

western blot, immunocytochemistry

Antibody

Mouse Anti Maltose-Binding protein

Maltose

MBP Antibody; Maltose-Binding protein, Mouse Antibody

myelin basic protein isoform 1; Myelin basic protein; Golli-MBP; myelin basic protein; myelin A1 protein; myelin membrane encephalitogenic protein; myelin basic protein; Myelin A1 protein; Myelin membrane encephalitogenic protein

MBP

MBP; MBP; MBP

MBP_HUMAN

Monoclonal

197

Mouse monoclonal antibody against the amino acid stretch of KDPRIAA (the amino acid residue 339 to 345) of the periplasmic maltose-binding protein (MBP) [Escherichia coli] (Accession #: AAB59056).

Store unopened antibody at 4 degree C or lower. Under these conditions, there is no significant loss in product performance one year from the purchase date. The reconstituted antibody is stable for at least two months when stored at 4 degree C. For long term storage, it is recommended that aliquots of the antibody are frozen at -20 degree C (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use.

Western Blot (WB), Immunofluorescence (IF)

Recommended: working dilution on Western bloting 1:500. 60 minutes primary antibody incubates at 25 degree C. Immunofluorescence: Typical working dilution on frozen sections 1:200. 60 minutes primary antibody incubates at 25 degree C.

Antigen used for immunization: The malE gene is inserted in a pMAL vector (Maina et al., 1988) and then expressed in E. coli JM109. The purified recombinant MBP protein was then used for mouse immunization. Storage Buffer: Theammonium sulfate-precipitated ascites fluid is in PBS + 1% BSA & then lyophilized.

MONOCLONAL/POLYCLONAL ANTIBODIES, MISCELLANEOUS ANTIBODIES; Monoclonal Antibodies

Introduction: Maltose Binding Protein (MBP) is a part of the maltose/maltodextrin system of Eschericia coli bacteria, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. Maltose binding protein MalE,MBP, is part of the maltodextrin transport system in E. coli. The maltodextrins diffuse through the LamB pore located in the outer membrane into the periplasm. The sugar is bound to MalE which prevents it to diffuse back outside. MalE passes the sugar to a complex of MalF and MalG in the cytoplasmic membrane where active tranport is mediated by the ATPase MalK. MBP molecular weight is 40,622 dalton. There are two domains in the protein with similar secondary structure (central pleated sheets are surrounded by helices). The domains are connected by three bridges, between the domains is a deep groove in which the sugar is bound. All hydroxyl groups of the sugar are fixed by sidechains of the protein by hydrogen bonds. Some water molecules are part of the hydrogen bridge network.

68509930

NP_001020252.1

NM_001025081.1

17,343 Da

Glial Cell Differentiation Pathway 698758!!MAPK Cascade Pathway 198834!!Neural Crest Differentiation Pathway 672460!!Spinal Cord Injury Pathway 739007

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]

0.02 mg

325 USD