product summary
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company name :
MyBioSource
product type :
antibody
product name :
Mouse Anti FLAG peptide
catalog :
MBS140047
quantity :
0.5 mg
price :
230 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
NYRFLAG
application :
ELISA, enzyme immunoassay
more info or order :
product information
catalog number :
MBS140047
products type :
Antibody
products full name :
Mouse Anti FLAG peptide
products short name :
FLAG peptide
products name syn :
FLAG Antibody; FLAG peptide, Mouse Antibody; FLAG peptide
other names :
FlaG; FlaG; FlaG
products gene name :
FLAG
other gene names :
flaG
uniprot entry name :
C1DCR6_LARHH
clonality :
Monoclonal
isotype :
Mouse IgG1/kappa
clone :
NYRFLAG
host :
Mouse
sequence length :
137
purity :
Protein A & Ion exchange column.
concentration :
1mg/ml in PBS (after reconstitution).
storage stability :
In lyophilized form, for long periods, store at 4 degree C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20 degree C. Antibody is shipped lyophilized at ambient temperature.
tested application :
ELISA (EIA)
app notes :
By direct ELISA, 1:20,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig.
other info1 :
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
other info2 :
Reconstitution: Reconstitute with H20 to get a 1 mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Note: The antibody was produced in BALB/c mice. Will recognize FLAG (DYKDDDDK) conjugated at either the amino or carboxy terminus. Can be used bound to various resins for affinity purifications. Immunogen: Flag-conjugated proteins.
products categories :
MONOCLONAL/POLYCLONAL ANTIBODIES, MISCELLANEOUS ANTIBODIES; Monoclonal Antibodies
products description :
Introduction: A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody. denoted as M1. binds the peptide in the presence of bivalent metal cations. preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide.The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins.
ncbi gi num :
226941620
ncbi acc num :
YP_002796694.1
ncbi gb acc num :
NC_012559.1
ncbi mol weight :
15,150 Da
size1 :
0.5 mg
price1 :
230 USD
size2 :
1 mg
price2 :
350
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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