Mouse Complement C3 ELISA kit | MyBioSource MBS135930 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Complement C3 ELISA kit

catalog :

MBS135930

quantity :

1 Kit

price :

445 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS135930

products type :

ELISA Kit

products full name :

Mouse Complement C3 ELISA kit

products short name :

[Complement C3]

other names :

[complement C3; Complement C3; complement C3; complement factor 3; complement component 3d; acylation stimulating protein; complement component 3; HSE-MSFCleaved into the following 12 chains:Complement C3 beta chain; C3-beta-c; C3bc; Complement C3 alpha chain; C3a anaphylatoxin; Acylation stimulating protein; ASPAlternative name(s):C3adesArg]

other gene names :

[C3; C3; ASP; Plp; HSE-MSF; AI255234; C3bc; ASP]

uniprot entry name :

CO3_MOUSE

reactivity :

Mouse

sequence length :

1663

specificity :

This assay recognizes total a2-antiplasmin in mouse plasma. Pooled normal plasma from rabbit, sheep, rat, pig, dog, and human was assayed and no significant cross-reactivity was observed.

form :

Complete kit

storage stability :

Recommended Storage: 4 degree C. Minimum Shelf Life: 12 months from manufacture, see label for expiration date. Shipping Condition: Cold pack

other info1 :

Samples: Mouse Plasma.

other info2 :

Intra-assay Precision: These studies are currently in progress. Please contact us for more information. Inter-assay Precision: These studies are currently in progress. Please contact us for more information.

products categories :

COMPLEMENT

products description :

Intended Uses: This mouse a2-antiplasmin assay is for the quantitative determination of total a2-antiplasmin in mouse plasma. For research use only. Principle of the Assay: Total mouse a2-antiplasmin will bind to the affinity purified capture antibody coated on the microtiter plate. Complexed and free a2-antiplasmin will react with the antibody on the plate. After appropriate washing steps, biotin labeled polyclonal anti-mouse a2-antiplasmin primary antibody binds to the captured protein. Excess antibody is washed away and bound antibody is reacted with streptavidin conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse a2-antiplasmin. Color development is proportional to the concentration of a2-antiplasmin in the samples!!Background/Introduction: a2-antiplasmin is the major circulating inhibitor of plasmin. It plays a role in the regulation of intravascular fibrinolysis [1,2]. Decreased levels of a2-antiplasmin may play an important role in the increased capacity of the fibrinolytic function and may be beneficial in the treatment of thrombotic diseases, acute pulmonary embolism, and hepatic repair [3,4,6,7].

ncbi gi num :

126518317

ncbi acc num :

NP_033908.2

ncbi gb acc num :

NM_009778.2

uniprot acc num :

P01027

ncbi mol weight :

60,952 Da

ncbi pathways :

Activation Of C3 And C5 Pathway (1110737); Adaptive Immune System Pathway (1110669); Alternative Complement Activation Pathway (1110736); Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795); Class A/1 (Rhodopsin-like Receptors) Pathway (1110499); Complement Activation, Classical Pathway (198379); Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484)

ncbi summary :

This gene encodes complement protein C3 which plays a central role in the classical, alternative and lectin activation pathways of the complement system. The encoded preproprotein undergoes a multi-step processing to generate various functional peptides. Mice deficient in the encoded protein fail to clear bacteria from the blood stream upon infection, display diminished airway hyperresponsiveness and lung eosinophilia upon allergen-induced pulmonary allergy, and develop severe lung injury after deposition of IgG immune complexes. Deficiency of the homolog of the encoded protein in humans was found to be associated with increased susceptibility to infections, age-related macular degeneration, and atypical hemolytic uremic syndrome. [provided by RefSeq, Mar 2015]

uniprot summary :

C3: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Defects in C3 are the cause of complement component 3 deficiency (C3D). A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis. Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin- containing structure known as Bruch membrane. Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage. Protein type: Secreted; Inhibitor; Secreted, signal peptide. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; endopeptidase inhibitor activity; cofactor binding; lipid binding; C5L2 anaphylatoxin chemotactic receptor binding. Biological Process: positive regulation of developmental growth; immune system process; complement activation, alternative pathway; fatty acid metabolic process; complement activation; positive regulation of angiogenesis; positive regulation of activation of membrane attack complex; positive regulation of phagocytosis; positive regulation of G-protein coupled receptor protein signaling pathway; positive regulation of type IIa hypersensitivity; innate immune response; positive regulation of protein amino acid phosphorylation; lipid metabolic process; blood coagulation; inflammatory response; complement activation, classical pathway

size1 :

1 Kit

price1 :

445 USD

size2 :

5 Kits

price2 :

1850

more info or order :

MyBioSource product webpage