Chromogenic substrate for plasmin | MyBioSource MBS135890 product information

product summary

company name :

MyBioSource

product type :

chemical

product name :

Chromogenic substrate for plasmin

catalog :

MBS135890

quantity :

25 mg

price :

280 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS135890

products type :

Substrate

products full name :

Chromogenic substrate for plasmin

products short name :

Chromogenic substrate for plasmin

other names :

plasminogen isoform 1; Plasminogen; plasminogen; plasminogen

products gene name :

PLG

other gene names :

PLG; PLG

uniprot entry name :

PLMN_HUMAN

host :

Chemical

sequence length :

810

purity :

98.9% by HPLC

form :

Lyophilized powder

concentration :

Lyophilized

storage stability :

Store at -70 degree C. Shelf Life: 3 years from delivery. Shipping: Cold Packs

other info1 :

Aliquot: 1 x 25 mg with 60 mg mannitol as bulking agent. Volume: Resuspend to 10mM in I mM HCl. Formula: H-D-Val-Leu-Lys-pNA . 2HCI. Mr: 551.6 Da

other info2 :

Buffer: Dilute to 0.2mM in assay buffer

products categories :

Chromogenic Substrates; Chromogenic Substrate for plasmin

products description :

The plasmin substrate provide by our company is chemically and functionally identical to Chromogenix S-2251. Each 25 mg of substrate is lyophilized with 60 mg mannitol as a bulking agent. For best results add 4.5 ml of I mM HCI directly to vial to make a 10mM stock solution then aliquot and freeze. Dilute 1:50 in PBS or TBS to make an 0.2mM working solution. Add 0.1 ml test sample to 0.9 ml substrate and monitor color development at 405nm.

ncbi gi num :

4505881

ncbi acc num :

NP_000292.1

ncbi gb acc num :

NM_000301.3

uniprot acc num :

P00747

ncbi mol weight :

555.1

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Degradation Of The Extracellular Matrix Pathway (576263); Dissolution Of Fibrin Clot Pathway (106061); Extracellular Matrix Organization Pathway (576262); Hemostasis Pathway (106028)

ncbi summary :

The protein encoded by this gene is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasmin dissolves fibrin in blood clots and is an important protease in many other cellular processes while angiostatin inhibits angiogenesis. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009]

uniprot summary :

Plasminogen: Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. Defects in PLG are the cause of plasminogen deficiency (PLGD). PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa. Belongs to the peptidase S1 family. Plasminogen subfamily. Protein type: EC 3.4.21.7; Secreted; Protease; Secreted, signal peptide; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 6q26. Cellular Component: extracellular space; extrinsic to external side of plasma membrane; cell surface; extracellular region; plasma membrane. Molecular Function: protein domain specific binding; protein binding; serine-type peptidase activity; serine-type endopeptidase activity; apolipoprotein binding; receptor binding. Biological Process: platelet activation; extracellular matrix organization and biogenesis; tissue remodeling; myoblast differentiation; muscle maintenance; negative regulation of cell proliferation; extracellular matrix disassembly; fibrinolysis; cellular protein metabolic process; negative regulation of fibrinolysis; platelet degranulation; proteolysis involved in cellular protein catabolic process; tissue regeneration; positive regulation of fibrinolysis; blood coagulation; transmembrane transport. Disease: Plasminogen Deficiency, Type I

size1 :

25 mg

price1 :

280 USD

size2 :

100 mg

price2 :

755

more info or order :

MyBioSource product webpage