protein

Mouse Prorenin, C-terminal 8x His tag

MBS135767

0.1 mg

365 USD

Protein

Mouse Prorenin, C-terminal 8x His tag

Prorenin, C-terminal 8x His tag

renin-1; Renin-1; renin-1; renin b; kidney renin; aspartyl-protease; angiotensinogenase; renin 1 structural; Angiotensinogenase; Kidney renin

REN1

Ren1; Ren1; Ren; Rnr; Rn-1; Ren-1; Ren-A; Ren1c; Ren1d; D19352; Ren; Ren-1

RENI1_MOUSE

Human Embryonic Kidney cells

402

>95% by SDS-PAGE analysis

Frozen liquid

0.9 mg/ml

Store at -70 degree C. Shelf Life: 3 years from delivery

Aliquot: 1 x 0.1mg. Volume: 0.111ml

Buffer: 50 mM Tris; pH 8.0. Extinction Coefficient: 1.1

Renin/Prorenin; Mouse Prorenin

Recombinantly produced in HEK cell culture and purified by chelated metal affinity chromatography. Contains a 8X-Histidine tag at C terminus for purification. Resistant to activation to renin by trypsin digestion. Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Prorenin ezhibits a low level of enzymatic activity relative to renin which is generated from prorenin by proteolytic cleavage of the first ~43 amino acids at the N- terminus. This so called prosegment appears to block the full enzymatic potential of the active site (1). Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects (2).

13676837

NP_112469.1

NM_031192.3

P06281

44,000

ACE Inhibitor Pathway (198320); Metabolism Of Angiotensinogen To Angiotensins Pathway (765637); Metabolism Of Proteins Pathway (765593); Peptide Hormone Metabolism Pathway (765634); Renin-angiotensin System Pathway (83272); Renin-angiotensin System Pathway (486)

REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Protease; EC 3.4.23.15. Cellular Component: extracellular space; membrane; cytoplasm; extracellular region; intracellular. Molecular Function: peptidase activity; insulin-like growth factor receptor binding; hydrolase activity; endopeptidase activity; aspartic-type endopeptidase activity; receptor binding. Biological Process: response to drug; response to cAMP; establishment of blood-nerve barrier; hormone-mediated signaling; cell maturation; drinking behavior; renin-angiotensin regulation of aldosterone production; angiotensin maturation; proteolysis; response to organic substance; regulation of MAPKKK cascade; regulation of blood pressure; renin-angiotensin regulation of blood volume; kidney development

0.1 mg

365 USD

1 mg

2690