protein

Bovine plasmin

MBS135756

1 mg

285 USD

Protein

Bovine plasmin

Bovine plasmin

plasminogen; Plasminogen; plasminogen; lipoprotein, Lp(a)

PLG

PLG; PLG; LPA

PLMN_BOVIN

Bovine plasma

812

>95% by SDS-PAGE analysis

Frozen liquid

1.12 mg/ml

Store at -70 degree C. Shelf Life: 3 years from delivery. Shipping: Dry Ice.

Volume: 0.089ml. Aliquot: 10 x 0.1mg

Buffer: 0.1M HEPES; 0.1M NaCl; pH 7.4. Extinction Coefficient: 1.69

Plasminogen/Plasmin/Antiplasmin; Bovine Plasminogen and Plasmin

Prepared from plasminogen by activation with immobilized human uPA. 100 percent functionally active plasmin is purified from the activation reaction by immobilized SBTI. Plasmin will undergo rapid auto proteolysis in the absence of benzamidine and should be used quickly once thawed. Aliquot to avoid freeze-thaw cycles. Partial cleavage of bovine plasmin between kringles 3 & 4 occurs upon activation, production a low molecular weight band. This midiplasmin is 100 percent active (Christensen et al. Biochem J. 305 (Pt 1): 97-102, 1995).

27806815

NP_776376.1

NM_173951.2

P06868

85,000

Complement And Coagulation Cascades Pathway (84312); Complement And Coagulation Cascades Pathway (484); Influenza A Pathway (217172); Influenza A Pathway (217150); Neuroactive Ligand-receptor Interaction Pathway (84292); Neuroactive Ligand-receptor Interaction Pathway (462); Staphylococcus Aureus Infection Pathway (172831); Staphylococcus Aureus Infection Pathway (171867)

Function: Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells . By similarity. Catalytic activity: Preferential cleavage: Lys- -Xaa > Arg- -Xaa; higher selectivity than trypsin. Converts fibrin into soluble products. Enzyme regulation: Converted into plasmin by plasminogen activators, both plasminogen and its activator being bound to fibrin. Cannot be activated with streptokinase. Subunit structure: Interacts with CSPG4 and AMOT. Interacts (via the Kringle domains) with HRG; the interaction tethers PLG to the cell surface and enhances its activation . By similarity. Subcellular location: Secreted . By similarity. Note: Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface . By similarity. Domain: Kringle domains mediate interaction with CSPG4 . By similarity. Post-translational modification: N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide which is modified with up to 2 sialic acid residues (microheterogeneity).In the presence of the inhibitor, the activation involves only cleavage after Arg-583, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide . By similarity. Miscellaneous: Plasmin is inactivated by alpha-2-antiplasmin immediately after dissociation from the clot. Sequence similarities: Belongs to the peptidase S1 family. Plasminogen subfamily.Contains 5 kringle domains.Contains 1 PAN domain.Contains 1 peptidase S1 domain.

1 mg

285 USD

10 mg

1815