Human tissue plasminogen activator, >85% single chain | MyBioSource MBS135727 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Human tissue plasminogen activator, >85% single chain

catalog :

MBS135727

quantity :

0.1 mg

price :

300 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS135727

products type :

Protein

products full name :

Human tissue plasminogen activator, >85% single chain

products short name :

[tissue plasminogen activator, >85% single chain]

other names :

[tissue-type plasminogen activator isoform 1 preproprotein; Tissue-type plasminogen activator; tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA); plasminogen activator, tissue]

products gene name :

[PLAT]

other gene names :

[PLAT; PLAT; TPA; T-PA; t-PA; t-plasminogen activator; tPA]

uniprot entry name :

TPA_HUMAN

host :

CHO cells

sequence length :

562

purity :

>95% by SDS-PAGE analysis

form :

Frozen liquid

concentration :

3.6 mg/ml

storage stability :

Store at -70 degree C. Shelf Life: 3 years from delivery

other info1 :

Aliquot: 1 x 1.0mg. Volume: 0.278ml. Specific Activity: 467,295 IU/mgIU/mg (Relative to WHO International Standard for Human Recombinant Tissue Plasminogen Activator, NIBSC 98/714)

other info2 :

Buffer: 0.4M HEPES; 0.1M NaCl; pH 7.4. Extinction Coefficient: 1.9

products categories :

Tissue Plasminogen Activator (tPA); Human tPA

products description :

Human tissue-type plasminogen activator (tPA) is a 527 amino acid glycoprotein. Synthesized from cDNA from a human melanoma cell line. It is recombinantly produced in Chinese Hamster Ovary (CHO) cells. Fully active, >85percent single chain.

ncbi gi num :

4505861

ncbi acc num :

NP_000921.1

ncbi gb acc num :

NM_000930.3

uniprot acc num :

P00750

ncbi mol weight :

70,000

ncbi pathways :

Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Dissolution Of Fibrin Clot Pathway (106061); Endochondral Ossification Pathway (198812); Hemostasis Pathway (106028); Selenium Pathway (198825); Senescence And Autophagy Pathway (198780); Signal Transduction Pathway (477114)

ncbi summary :

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

PLAT: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Motility/polarity/chemotaxis; Cytoskeletal; Vesicle; Secreted, signal peptide; EC 3.4.21.68; Protease. Chromosomal Location of Human Ortholog: 8p12. Cellular Component: extracellular matrix; extracellular space; cell surface; apical part of cell; cytoplasm; extracellular region; synapse; secretory granule. Molecular Function: protein binding; serine-type endopeptidase activity. Biological Process: negative regulation of proteolysis; synaptic transmission, glutamatergic; response to peptide hormone stimulus; fibrinolysis; response to cAMP; smooth muscle cell migration; response to hypoxia; regulation of synaptic plasticity; protein modification process; platelet-derived growth factor receptor signaling pathway; proteolysis; blood coagulation; transmembrane transport; plasminogen activation. Disease: Thrombophilia, Familial, Due To Decreased Release Of Tissue Plasminogen Activator

size1 :

0.1 mg

price1 :

300 USD

size2 :

1 mg

price2 :

690

more info or order :

MyBioSource product webpage