ELISA/assay

Active rat PAI-1 functional assay ELISA Kit

MBS135538

1 Kit

540 USD

ELISA Kit

Active rat PAI-1 functional assay ELISA Kit

[PAI-1 functional assay]

[plasminogen activator inhibitor 1; Plasminogen activator inhibitor 1; plasminogen activator inhibitor 1; PAI; PAI-1; serpin E1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, member 1; serine (or cysteine) peptidase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; Endothelial plasminogen activator inhibitor; Serpin E1]

[SERPINE1]

[Serpine1; Serpine1; Pai1; PAI1A; Planh; Pai1aa; RATPAI1A; Pai1; Planh1; PAI; PAI-1]

PAI1_RAT

General

402

These studies are currently in progress. Please contact us for more information.

Complete Kit

Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat plasma. Sensitivity: 0.011 ng/ml (calculated by determining the OD of 20 reps of So and 20 reps of the low standard)

Intra-assay Precision: High concentration %CV=3.5%, Medium concentration %CV=4.9%, Low concentration %CV=2.8% (calculated by running 24 reps of each concentration in one assay). Inter-assay Precision: These studies are currently in progress. Please contact us for more information.

Plasminogen Activator Inhibitor 1 (PAI-1); PAI-1 ELISA Kits

Intended Uses: Rat PAI-1 activity assay is intended for the quantitative determination of active plasminogen activator inhibitor type 1 in rat plasma. For research use only. Principle of the Assay: Functionally active PAI-1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. Unbound PAI-1 samples are washed away and an anti-PAI-1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the original active PAI-1 present in the samples, is then reacted with the horseradish peroxidase secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI-1 in the sample. Background: Plasminogen activator inhibitor-1 (PAI-1) is a serine protease inhibitor that is an important regulator of fibrinolysis and extracellular matrix turnover [1,2,6]. PAI-1 may be important in hepatocyte growth and proliferation in vivo. Increased PAI-1 levels may increase the risk for myocardial infarction, atherosclerosis, and retenosis [3,4]. Increased PAI-1 levels may also play an important role of the development and pathogenesis of diabetic nephropathy [5]. Decreased levels may reduce thrombotic events [7].

6981332

NP_036752.1

NM_012620.1

P20961

45,010 Da

Adipogenesis Pathway (198479); BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway (775963); Blood Clotting Cascade Pathway (198493); Brain Derived Neurotrophic Factor Pathway (755433); Chagas Disease (American Trypanosomiasis) Pathway (147806); Chagas Disease (American Trypanosomiasis) Pathway (147795); Circadian Clock Pathway (775962); Complement And Coagulation Cascades Pathway (198461); Complement And Coagulation Cascades Pathway (83465); Complement And Coagulation Cascades Pathway (484)

mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator; may promote neuronal survival [RGD, Feb 2006]

SERPINE1: a secreted protein that acts as bait for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted. Cellular Component: extracellular matrix; extracellular space; extracellular region. Molecular Function: serine-type endopeptidase inhibitor activity; protease binding; receptor binding. Biological Process: positive regulation of blood coagulation; wound healing; response to lipopolysaccharide; positive regulation of coagulation; decidualization; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; glucose homeostasis; mast cell activation; positive regulation of receptor-mediated endocytosis; positive regulation of interleukin-8 production; positive regulation of fibroblast proliferation; negative regulation of fibrinolysis; tissue regeneration; angiogenesis; regulation of receptor activity; chronological cell aging; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; female gonad development; positive regulation of keratinocyte migration; negative regulation of proteolysis; regulation of angiogenesis; regulation of cell proliferation; response to starvation; response to reactive oxygen species; positive regulation of angiogenesis; response to hyperoxia; cellular response to insulin stimulus; response to estrogen stimulus; response to cytokine stimulus; positive regulation of inflammatory response

1 Kit

540 USD

5 Kits

1980