catalog number :
MBS135512
products type :
ELISA Kit
products full name :
Rabbit Immunoglobulin G ELISA Kit
products short name :
Rabbit Immunoglobulin G
storage stability :
Store at 4 degree C. Shelf Life: 6 months from manufacture, see label for expiration date
other info1 :
Samples: Serum, plasma, hybridoma cell supernatants, ascites or other biological fluids
products categories :
Animal Immunoglobulins; Rabbit Immunoglobulin ELISA Kits
products description :
Intended Uses: This rabbit Immunoglobulin G (IgG) antigen assay is intended for the quantitative determination of total rabbit IgG antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The assay does not distinguish IgG subclasses. Principle of the Assay: Rabbit IgG will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-rabbit IgG antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rabbit IgG. Color development is proportional to the concentration of IgG in the samples. Background: IgG is the most abundant immunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in human serum.