catalog number :
MBS135498
products type :
ELISA Kit
products full name :
Rat Prolactin ELISA Kit
products short name :
Prolactin
other names :
prolactin; Prolactin; prolactin; prolactin family 1, subfamily a, member 1; prolactin
other gene names :
Prl; Prl; PRLB; Prol; PRLSD1; Prl1a1; RNPROL; RATPRLSD1; PRL
uniprot entry name :
PRL_RAT
storage stability :
Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
other info1 :
Samples: Rat plasma
products categories :
Prolactin/Prolactin Receptor; Prolactin ELISA Kits
products description :
Intended Uses: This rat prolactin antigen assay is intended for the quantitative determination of prolactin antigen in rat plasma. Principle of the Assay: Rat prolactin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotinlyated anti-rat prolactin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total prolactin present in the samples, is reacted with peroxidase-conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development which is measured at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat prolactin.Color development is proportional to the concentration of prolactin in the samples. Background: Rat prolactin (PRL) is a 197 aa, 23kD peptide hormone [1] that is secreted primarily by the pituitary gland in both males and females, though its major roles are in pregnancy and lactation [2,3]. Prolactin may have a role in breast cancer development, with higher prolactin levels correlating with postmenopausal breast cancer risk [4].
ncbi acc num :
NP_036761.1
ncbi gb acc num :
NM_012629.1
ncbi mol weight :
25,753 Da
ncbi pathways :
Amyloids Pathway (775645); Cytokine Signaling In Immune System Pathway (776309); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Disease Pathway (775616); Growth Hormone Receptor Signaling Pathway (776326); Immune System Pathway (776211); Jak-STAT Signaling Pathway (83469); Jak-STAT Signaling Pathway (488); Neuroactive Ligand-receptor Interaction Pathway (83445)
ncbi summary :
induces lactation; may facilitate decidual growth and placentation; promotes prostate epithelial cell proliferation [RGD, Feb 2006]
uniprot summary :
prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family. Protein type: Motility/polarity/chemotaxis; Secreted; Cytokine; Secreted, signal peptide. Cellular Component: extracellular region; secretory granule. Molecular Function: prolactin receptor binding; hormone activity. Biological Process: circadian rhythm; response to drug; lactation; maternal behavior; positive regulation of JAK-STAT cascade; regulation of multicellular organism growth; response to lipopolysaccharide; response to organic cyclic substance; response to estradiol stimulus; cellular response to hormone stimulus; ovulation cycle; regulation of JAK-STAT cascade; response to ethanol; mammary gland development; positive regulation of cell proliferation; parturition; regulation of ossification; multicellular organismal response to stress; response to nutrient; positive regulation of epithelial cell proliferation; STAT protein nuclear translocation