protein

Recombinant Rat Cryptochrome-2 (Cry2)

MBS1346594

0.05 mg (E-Coli)

1015 USD

Recombinant Protein

Recombinant Rat Cryptochrome-2 (Cry2)

[Cryptochrome-2 (Cry2)]

[Cryptochrome-2; Cryptochrome-2; cryptochrome-2; cryptochrome circadian regulator 2]

[Cry2]

[Cry2]

[Cry2; Cry2]

E Coli or Yeast or Baculovirus or Mammalian Cell

[1-594aa, full length protein]

594

MAAAAVVAATVPVQSMGADGASSVHWFRKGLRLHDNPAL
LAAVRGARCVRCVYILDPWFAASSSVGINRWRFLLQSLE
DLDTSLRKLNSRLFVVRGQPADVFPRLFKEWGVTRLTFE
YDSEPFGKERDAAIMKMAKEAGVEVVTENSHTLYDLDRI
IELNGQKPPLTYKRFQALISRMELPKKPVGAVSSQHMEN
CRAEIQENHDDTYGVPSLEELGFPTEGLGPAVWQGGETE
ALVRLDKHLERKAWVANYERPRMNANSLLASPTGLSPYL
RFGCLSCRLFYYRLWDLYRKVKRNSTPPLSLFGQLLWRE
FFYTAATNNPRFDRMEGNPICIQIPWDRNPEALAKWAEG
KTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWV
SWESGVRVFDELLLDADFSVNAGSWMWLSCSAFFQQFFH
CYCPVGFGRRTDPSGDYIRRYLPKLKGFPSRYIYEPWNA
PESVQKAANCIIGVDYPRPIVNHAETSRLNIERMKQIYQ
QLSRYRGLCLWASVPSCVEDLSHPVAEPGSSQAGSISNT
GPRPLSSGPASPKRKLEAAEEPPGEELSKRARVTVTQMP
AQEPPSKDS

>85% (SDS-PAGE)

Liquid containing glycerol

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Testing Data

Species: Rattus norvegicus (Rat)

Storage Buffer: Tris-based buffer, 50% glycerol

81867613

Q923I8.1

Q923I8

67,223 Da

Circadian Clock Pathway (573763); Circadian Rhythm - Mammal Pathway (83476); Circadian Rhythm - Mammal Pathway (495); Diurnally Regulated Genes With Circadian Orthologs Pathway (198473)

This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]

Transcriptional repressor which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK NPAS2-ARNTL/BMAL1 ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. CRY1 and CRY2 have redundant functions but also differential and selective contributions at least in defining the pace of the SCN circadian clock and its circadian transcriptional outputs. Less potent transcriptional repressor in cerebellum and liver than CRY1, though less effective in lengthening the period of the SCN oscillator. Seems to play a critical role in tuning SCN circadian period by opposing the action of CRY1. With CRY1, dispensable for circadian rhythm generation but necessary for the development of intercellular networks for rhythm synchrony. May mediate circadian regulation of cAMP signaling and gluconeogenesis by blocking glucagon-mediated increases in intracellular cAMP concentrations and in CREB1 phosphorylation. Besides its role in the maintenance of the circadian clock, is also involved in the regulation of other processes. Plays a key role in glucose and lipid metabolism modulation, in part, through the transcriptional regulation of genes involved in these pathways, such as LEP or ACSL4. Represses glucocorticoid receptor NR3C1/GR-induced transcriptional activity by binding to glucocorticoid response elements (GREs). Represses the CLOCK-ARNTL/BMAL1 induced transcription of BHLHE40/DEC1 and NAMPT.

0.05 mg (E-Coli)

1015 USD

0.05 mg (Yeast)

1055

0.05 mg (Baculovirus)

1295

0.2 mg (E-Coli)

1370

0.2 mg (Yeast)

1440

0.5 mg (E-Coli)

1515

0.05 mg (Mammalian-Cell)

1535

0.5 mg (Yeast)

1630

0.1 mg (Baculovirus)

1870

1 mg (E-Coli)

2295

0.5 mg (Baculovirus)

2420

0.1 mg (Mammalian-Cell)

2475

1 mg (Yeast)

2570

1 mg (Baculovirus)

3725