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antibody

Rabbit Anti-BRCA1 (Ab-1423) Polyclonal Antibody

MBS132398

0.04 mg

140 USD

polyclonal

rabbit

mouse, chimpanzee, rat, human

immunohistochemistry

MBS132398

Antibody

Rabbit Anti-BRCA1 (Ab-1423) Polyclonal Antibody

BRCA1 (Ab-1423)

Anti BRCA1

BRCA1; Breast cancer type 1 susceptibility protein homolog; breast cancer type 1 susceptibility protein homolog; RING finger protein 53

BRCA1

BRCA1; BRCA1; RNF53

BRCA1_PANTR

Polyclonal

Rabbit IgG

Rabbit

Human, mouse, rat

1863

Rabbit Anti-BRCA1 (Ab-1423) Polyclonal Antibody detects endogenous levels of total BRCA1 protein.

The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.

1 mg/ml in phosphate buffered saline (without Mg2+ and Ca2+). pH 7.4. 150 mM NaCl. 0.02% sodium azide. and 50% glycerol

Store at -20 degree C/1 year

Immunohistochemistry

IHC: 1:50-1:100

Immunogen: Synthesized non-phosphopeptide derived from human BRCA1 around the phosphorylation site of serine 1423 (H-G-SP-Q-P). Antigen Species: Human

Conjugation: Unconjugated

Chromatin Transcription Pathway

37953275

Q9GKK8

207,899 Da

Androgen Receptor Signaling Pathway 199040!!Ubiquitin Mediated Proteolysis Pathway 83772!!Ubiquitin Mediated Proteolysis Pathway 466

Function: E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Subunit structure: Heterodimer with BARD1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. Interacts (via BRCT domains) with FAM175A/Abraxas and RBBP8. Associates with RNA polymerase II holoenzyme. Interacts with SMC1A and COBRA1/NELFB. Interacts (via BRCT domains) with phosphorylated BRIP1. Interacts with FANCD2 (ubiquitinated). Interacts with BAP1. Interacts with DCLRE1C/Artemis and CLSPN. Interacts with H2AFX (phosphorylated on 'Ser-140'). Interacts with CHEK1/CHK1. Interacts with BRCC3. Interacts (via BRCT domains) with ACACA (phosphorylated); the interaction prevents dephosphorylation of ACACA. Interacts with STK6/AURKA. Interacts with UBXN1. Part of a trimeric complex containing BRCA1, BRCA2 and PALB2. Interacts with PALB2 and this interaction is essential for its function in HRR. Interacts with BRCA2 only in the presence of PALB2 which serves as the bridging protein. Subcellular location: Nucleus. By similarity. Note: Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex. Domain: The BRCT domains recognize and bind phosphorylated pSXXF motif on proteins. The interaction with the phosphorylated pSXXF motif of FAM175A/Abraxas, recruits BRCA1 at DNA damage sites. By similarity.The RING-type zinc finger domain interacts with BAP1. Post-translational modification: Phosphorylation at Ser-308 by STK6/AURKA is required for normal cell cycle progression from G2 to mitosis. Phosphorylated in response to IR, UV, and various stimuli that cause checkpoint activation, probably by ATM or ATR. By similarity.Autoubiquitinated, undergoes 'Lys-6'-linked polyubiquitination. 'Lys-6'-linked polyubiquitination does not promote degradation. Sequence similarities: Contains 2 BRCT domains.Contains 1 RING-type zinc finger.

0.04 mg

140 USD