catalog number :
MBS130173
products full name :
Mouse Anti-TUG Monoclonal Antibody
products short name :
TUG
purity :
The antibody is purified by protein A affinity chromatography and resuspended in 10 mM PBS (pH 7.6) and 0.02% sodium azide.
concentration :
1 mg/ml in 10 mM PBS (pH 7.6) and 0.02% sodium azide
storage stability :
The antibody remains stable for one to two weeks if stored at 2-8 degree C. For long-term storage. aliquot and store at -20 degree C or below. Use before is one year from the date of receipt.
tested application :
ELISA, Western Blot
app notes :
Western Blot: 1:500-1:2,000. ELISA: proposed dilution 1:10,000. Determining optimal working dilutions by titration test
other info1 :
Immunogen: Ni-NTA purified truncated recombinant TUG expressed in E. coli strain BL21 (DE3)
other info2 :
Conjugation: Unconjugated
products description :
The TUG protein contains a UBX domain. for GLUT4. In truncated form. TUG acts in a dominant-negative manner to inhibit insulin-stimulated GLUT4 redistribution in Chinese hamster ovary cells and 3T3-L1 adipocytes. Full-length TUG forms a complex specifically with GLUT4. In 3T3-L1 adipocytes. this complex is present in unstimulated cells and is largely disassembled by insulin. Endogenous TUG is localized with the insulin-mobilizable pool of GLUT4 in unstimulated 3T3-L1 adipocytes. and is not mobilized to the plasma membrane by insulin.
products references :
Jonathan S.B. Natalie H. Adrienne E. Lodish Functional
cloning of TUG as a regulator of GLUT4 glucose transporter trafficking. Nature 2003.425:727 - 733.