GRM4 Polyclonal Antibody | MyBioSource MBS129767 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

GRM4 Polyclonal Antibody

catalog :

MBS129767

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using GRM4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

image 2 :

Immunofluorescence analysis of MCF-7 cells using GRM4 antibody. Blue: DAPI for nuclear staining.

image 3 :

Immunohistochemistry of paraffn-embedded rat brain using PHF19antibody (MBS129767) at dilution of 1:100(40x lens)

product information

catalog number :

MBS129767

products type :

Antibody

products full name :

GRM4 Polyclonal Antibody

products short name :

[GRM4]

products name syn :

[mGlu4; GPRC1D; MGLUR4]

other names :

[Metabotropic glutamate receptor 4; Metabotropic glutamate receptor 4; metabotropic glutamate receptor 4; glutamate receptor, metabotropic 4]

products gene name :

[GRM4]

other gene names :

[GRM4; GRM4; mGlu4; GPRC1D; MGLUR4; GPRC1D; MGLUR4; mGluR4]

uniprot entry name :

GRM4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

912

purity :

Affinity Purification

storage stability :

Store at -20°C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:100

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Immunohistochemistry of paraffin-embedded human breast using PHF19antibody (A7284) at dilution of 1:100(40x lens).

other info1 :

Source: Rabbit. Species: Human. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

other info2 :

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-290 of human GRM4 (NP_000832.1). Calculated MW: 83kDa/86kDa/87kDa/88kDa/101kDa. Observed MW: 110kDa

products categories :

Polyclonal

products description :

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Several transcript variants encoding different isoforms have been found for this gene.

ncbi gi num :

2495077

ncbi acc num :

Q14833.1

uniprot acc num :

Q14833

ncbi pathways :

Class C/3 (Metabotropic Glutamate/pheromone Receptors) Pathway (106381); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class C Metabotropic Glutamate, Pheromone Pathway (198839); Glutamatergic Synapse Pathway (213818); Glutamatergic Synapse Pathway (213811); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637)

ncbi summary :

uniprot summary :

mGluR4: metabotropic glutamate receptor 3, a G-protein coupled receptor for glutamate. L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. Protein type: Membrane protein, integral; Cell development/differentiation; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 3. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: presynaptic membrane; integral to plasma membrane; plasma membrane; terminal button; presynaptic active zone membrane; intracellular; cytoplasmic vesicle; dendritic shaft. Molecular Function: calmodulin binding; G-protein coupled receptor activity; group III metabotropic glutamate receptor activity; glutamate receptor activity; calcium-dependent protein binding. Biological Process: synaptic transmission; regulation of synaptic transmission, glutamatergic; positive regulation of MAPKKK cascade; activation of MAPK activity; neurotransmitter secretion; regulation of neuron apoptosis; learning; metabotropic glutamate receptor, adenylate cyclase inhibiting pathway

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage