protein

Recombinant Arabidopsis thaliana Protein ROS1 (ROS1), partial

MBS1285940

0.01 mg (E-Coli)

235 USD

Recombinant Protein

Recombinant Arabidopsis thaliana Protein ROS1 (ROS1), partial

[Protein ROS1 (ROS1), partial]

[Protein ROS1; EC=3.2.2.-; DEMETER-like protein 1; Repressor of silencing 1]

[protein ROS1; Protein ROS1; protein ROS1; DEMETER-like protein 1; Repressor of silencing 1]

[ROS1]

[ROS1; DML1; At2g36490; F1O11.12]

[DML1; ROS1; demeter-like 1; DML1; F1O11.12; F1O11_12; REPRESSOR OF SILENCING1; ROS1; DML1]

ROS1_ARATH

E Coli or Yeast or Baculovirus or Mammalian Cell

[1-320aa; Partial]

MEKQRREESSFQQPPWIPQTPMKPFSPICPYTVEDQYHS
SQLEERRFVGNKDMSGLDHLSFGDLLALANTASLIFSGQ
TPIPTRNTEVMQKGTEEVESLSSVSNNVAEQILKTPEKP
KRKKHRPKVRREAKPKREPKPRAPRKSVVTDGQESKTPK
RKYVRKKVEVSKDQDATPVESSAAVETSTRPKRLCRRVL
DFEAENGENQTNGDIREAGEMESALQEKQLDSGNQELKD
CLLSAPSTPKRKRSQGKRKGVQPKKNGSNLEEVDISMAQ
AAKRRQGPTCCDMNLSGIQYDEQCDYQKMHWLYSPNLQQ
GGMRYDAI

>=90%

Liquid containing glycerol

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

SDS-PAGE

Species: Arabidopsis thaliana (Mouse-ear cress)

Epigenetics and Nuclear Signaling

Bifunctional DNA glycosylase/lyase, which excises 5-methylcytosine (5-meC) and 5-hydroxymethylcytosine (5-hmeC), leaving an apyrimidinic (AP) site that is subsequently incised by the lyase activity . Generates 3'-phosphor-alpha,beta-unsaturated aldehyde (3'-PUA) as a primary 5-meC excision intermediate. Prevents DNA hypermethylation, specifically in the promoter of otherwise silenced loci. May be involved in DNA repair through its nicking activity on methylated DNA. Binds with similar affinity to both methylated and non-methylated DNA. Highly distributive behavior on DNA substrates containing multiple 5-meC residues. Involved with Pol IV in the remodeling of the 5S rDNA chromatin via DNA methylation modifications during the first days of development post-germination. Participates in UV-B induced- and oxidative DNA damage repair .

42569673

NP_181190.3

NM_129207.4

Q9SJQ6

5kD

A repressor of transcriptional gene silencing. Functions by demethylating the target promoter DNA. Interacts physically with RPA2/ROR1. In the ros1 mutants, an increase in methylation is observed in a number of gene promoters. Among the loci affected by ros1, a few (RD29A and At1g76930) are affected in cytosine methylation in all sequence contexts (CpG, CpNpG or CpNpN), although many others are affected primarily in non-CpG contexts.

Bifunctional DNA glycosylase/lyase, which excises 5-methylcytosine (5-meC) and 5-hydroxymethylcytosine (5-hmeC), leaving an apyrimidinic (AP) site that is subsequently incised by the lyase activity (PubMed:25240767). Generates 3'-phosphor-alpha,beta-unsaturated aldehyde (3'-PUA) as a primary 5-meC excision intermediate (PubMed:25228464). Prevents DNA hypermethylation, specifically in the promoter of otherwise silenced loci. May be involved in DNA repair through its nicking activity on methylated DNA. Binds with similar affinity to both methylated and non-methylated DNA. Highly distributive behavior on DNA substrates containing multiple 5-meC residues. Involved with Pol IV in the remodeling of the 5S rDNA chromatin via DNA methylation modifications during the first days of development post-germination. Participates in UV-B induced- and oxidative DNA damage repair (PubMed:24155752).

0.01 mg (E-Coli)

235 USD

0.05 mg (E-Coli)

320

0.1 mg (E-Coli)

535

0.2 mg (E-Coli)

855

0.5 mg (E-Coli)

1125

1 mg (E-Coli)

1725