Phospho-Bad-S136 Polyclonal Antibody | MyBioSource MBS128547 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Phospho-Bad-S136 Polyclonal Antibody

catalog :

MBS128547

quantity :

0.05 mL

price :

235 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts from HT29 cells using Phospho-Bad-S136 antibody. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

image 2 :

Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-Bad-S136 antibody.

product information

catalog number :

MBS128547

products type :

Antibody

products full name :

Phospho-Bad-S136 Polyclonal Antibody

products short name :

[Bad-S136]

products name syn :

[Phospho-Bad-S136: BBC2; BCL2L8]

other names :

[Bcl2-associated agonist of cell death; Bcl2-associated agonist of cell death; bcl2-associated agonist of cell death; bcl-2-binding component 6; bcl2 antagonist of cell death; bcl-xL/Bcl-2-associated death promoter; BCL2-associated agonist of cell death; Bcl-2-binding component 6; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death]

products gene name :

[Bad]

other gene names :

[Bad; Bad; Bbc2; AI325008; Bbc6; BAD]

uniprot entry name :

BAD_MOUSE

clonality :

polyclonal

isotype :

IgG

host :

rabbit

reactivity :

Human, Mouse

sequence length :

204

purity :

Affinity Purification

form :

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

storage stability :

Store at -20°C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1:500 - 1:2000. IHC: 1:50 - 1:100

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Calculated MW: 18kDa. Observed MW: 18kDa . Immunogen: A phospho specific peptide corresponding to residues surrounding S136 of human Bad.

products categories :

Phosphorylation

products description :

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

ncbi gi num :

2493288

ncbi acc num :

Q61337.1

uniprot acc num :

Q92934

ncbi pathways :

AKT Phosphorylates Targets In The Cytosol Pathway (1110363); Activation Of BAD And Translocation To Mitochondria Pathway (1111505); Activation Of BH3-only Proteins Pathway (1111504); Acute Myeloid Leukemia Pathway (83310); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (1110669); Alpha6-Beta4 Integrin Signaling Pathway (198373); Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Amyotrophic Lateral Sclerosis (ALS) Pathway (83296)

uniprot summary :

BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL. Protein type: Apoptosis. Cellular Component: mitochondrial outer membrane; mitochondrion; membrane; cytoplasm; intracellular; cytosol. Molecular Function: protein kinase B binding; protein binding; protein heterodimerization activity; phospholipid binding; caspase activator activity; protein kinase binding; lipid binding; protein phosphatase binding; protein phosphatase 2B binding. Biological Process: positive regulation of proteolysis; apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; regulation of caspase activity; cellular process regulating host cell cycle in response to virus; glucose homeostasis; positive regulation of apoptosis by virus; regulation of apoptosis; pore complex biogenesis; positive regulation of glucokinase activity; caspase activation; release of cytochrome c from mitochondria; cytokine and chemokine mediated signaling pathway; suppression by virus of host apoptosis; ADP metabolic process; positive regulation of insulin secretion; ATP metabolic process; glucose catabolic process; cell proliferation; positive regulation of T cell differentiation; induction of apoptosis via death domain receptors; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of B cell differentiation; regulation of mitochondrial membrane permeability; positive regulation of epithelial cell proliferation

size1 :

0.05 mL

price1 :

235 USD

size2 :

0.1 mL

price2 :

350

size3 :

0.2 mL

price3 :

525

more info or order :

MyBioSource product webpage