Phospho-CDKN1B-T187 Polyclonal Antibody | MyBioSource MBS128516 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Phospho-CDKN1B-T187 Polyclonal Antibody

catalog :

MBS128516

quantity :

0.05 mL

price :

235 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts from HeLa cells, using Phospho-P27 KIP T187 antibody (MBS128516). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

image 2 :

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-p27 KIP 1-T187 antibody.

product information

catalog number :

MBS128516

products type :

Antibody

products full name :

Phospho-CDKN1B-T187 Polyclonal Antibody

products short name :

[CDKN1B-T187]

products name syn :

[CDKN1B; CDKN4; KIP1; MEN1B; MEN4; P27KIP1; Cyclin-dependent kinase inhibitor 1B]

other names :

[Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; p27Kip1]

products gene name :

[CDKN1B]

other gene names :

[CDKN1B; CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1; KIP1]

uniprot entry name :

CDN1B_HUMAN

clonality :

polyclonal

isotype :

IgG

host :

rabbit

reactivity :

Human, Mouse, Rat

sequence length :

198

purity :

Affinity Purification

form :

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

storage stability :

Store at -20 degree C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1:500 - 1:1000. IHC: 1:50 - 1:100

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: A phospho specific peptide corresponding to residues surrounding T187 of human p27 KIP 1.

products categories :

Phosphorylation

products description :

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4).

ncbi gi num :

1168871

ncbi acc num :

P46527.1

uniprot acc num :

P46527

ncbi mol weight :

Calculated: 22kDa. Observed: 26kDa

ncbi pathways :

AKT Phosphorylates Targets In The Cytosol Pathway (106475); Adaptive Immune System Pathway (366160); AhR Pathway (755436); C-MYB Transcription Factor Network Pathway (138073); Cell Cycle Pathway (530733); Cell Cycle Checkpoints Pathway (105739); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463)

ncbi summary :

uniprot summary :

p27Kip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. May mediate TGF beta-induced g1 arrest. Its degradation is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Protein type: Protein kinase, regulatory subunit; Cell cycle regulation; Oncoprotein; Inhibitor. Chromosomal Location of Human Ortholog: 12p13.1-p12. Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol; endosome. Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; chaperone binding; protein complex binding; caspase activator activity; Hsp70 protein binding; protein phosphatase binding; transforming growth factor beta receptor, cytoplasmic mediator activity. Biological Process: negative regulation of kinase activity; response to peptide hormone stimulus; nerve growth factor receptor signaling pathway; positive regulation of microtubule polymerization; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; response to estradiol stimulus; negative regulation of cell proliferation; sensory perception of sound; positive regulation of cell proliferation; response to glucose stimulus; negative regulation of mitotic cell cycle; cell cycle arrest; inner ear development; potassium ion transport; epidermal growth factor receptor signaling pathway; caspase activation; response to drug; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; negative regulation of cyclin-dependent protein kinase activity; negative regulation of cell motility; response to amino acid stimulus; response to cadmium ion; response to hypoxia; positive regulation of protein catabolic process; autophagic cell death; innate immune response; negative regulation of phosphorylation; negative regulation of cell growth; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; G1/S transition of mitotic cell cycle. Disease: Multiple Endocrine Neoplasia, Type Iv

size1 :

0.05 mL

price1 :

235 USD

size2 :

0.1 mL

price2 :

350

size3 :

0.2 mL

price3 :

525

more info or order :

MyBioSource product webpage