antibody

CYP11A1 Polyclonal Antibody

MBS128059

0.05 mL

285 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunocytochemistry

Antibody

CYP11A1 Polyclonal Antibody

CYP11A1

CYP11A; CYPXIA1; P450SCC

Cholesterol side-chain cleavage enzyme, mitochondrial; Cholesterol side-chain cleavage enzyme, mitochondrial; cholesterol side-chain cleavage enzyme, mitochondrial; steroid 20-22-lyase; cytochrome P450 11A1; cytochrome P450(scc); cytochrome P450C11A1; cholesterol 20-22 desmolase; cholesterol monooxygenase (side-chain cleaving); cytochrome P450, subfamily XIA (cholesterol side chain cleavage); cytochrome P450, family 11, subfamily A, polypeptide 1; CYPXIA1; Cholesterol desmolase; Cytochrome P450 11A1; Cytochrome P450(scc)

CYP11A1

CYP11A1; CYP11A1; CYP11A; CYPXIA1; P450SCC; CYP11A

CP11A_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

521

Affinity Purification

1mg/ml

Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB), Immunofluorescence (IF)

WB: 1:500 - 1:2000. IF: 1:20 - 1:50

Species: Human. Immunogen: Recombinant Protein

Immunogen: Recombinant protein of human CYP11A1. Calculated Molecular Weight: 60kDa. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Polyclonal

The formation of biologically active steroid hormones is significantly influenced by the motrochondrial cytochrome P450 enzyme, chrolesterol side-chain cleavage. CYP11A1 functions in catalyzing the first step of steroid biosynthesis under modulation of cAMP.It ia also known as cytochrome P450C11A1, cytochrome P450scc and cytochrome P450, subfamily XIA. Expression of CYP11A1 has been a useful tool for monitoring the differentiation state of cells from varius endocrine tissues. Identification of CYP11A1 expression by 13363-1-AP detected a ~45kd band in human placenta, with respect to the reported band at 49-52kd by Roby, et al and Durkee, et al.

143811381

P05108.2

P05108

521

Biological Oxidations Pathway (105698); Corticotropin-releasing Hormone Pathway (920957); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway (1127640); Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway (1127641); Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway (1127642); Defective CYP17A1 Causes Adrenal Hyperplasia 5 (AH5) Pathway (1127643); Defective CYP19A1 Causes Aromatase Excess Syndrome (AEXS) Pathway (1127644); Defective CYP1B1 Causes Glaucoma Pathway (1127645); Defective CYP21A2 Causes Adrenal Hyperplasia 3 (AH3) Pathway (1127646)

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]

CYP11A1: Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone. Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46,XY sex reversal (AICSR). A rare disorder that can present as acute adrenal insufficiency in infancy or childhood. ACTH and plasma renin activity are elevated and adrenal steroids are inappropriately low or absent; the 46,XY patients have female external genitalia, sometimes with clitoromegaly. The phenotypic spectrum ranges from prematurity, complete underandrogenization, and severe early-onset adrenal failure to term birth with clitoromegaly and later-onset adrenal failure. Patients with congenital adrenal insufficiency do not manifest the massive adrenal enlargement typical of congenital lipoid adrenal hyperplasia. Belongs to the cytochrome P450 family. Protein type: Mitochondrial; Lipid Metabolism - C21-steroid hormone; Oxidoreductase; EC 1.14.15.6. Chromosomal Location of Human Ortholog: 15q23-q24. Cellular Component: mitochondrial crista; mitochondrion; mitochondrial matrix; perikaryon. Molecular Function: cholesterol monooxygenase (side-chain-cleaving) activity; iron ion binding; cholesterol binding; heme binding. Biological Process: response to alkaloid; steroid metabolic process; maternal process involved in pregnancy; dibenzo-p-dioxin metabolic process; response to insecticide; estrogen biosynthetic process; response to L-ascorbic acid; response to salt stress; response to vitamin E; Leydig cell differentiation; biphenyl metabolic process; response to gamma radiation; cerebellum development; response to corticosterone stimulus; response to drug; cholesterol metabolic process; progesterone biosynthetic process; fractalkine metabolic process; phthalate metabolic process; hippocampus development; mating behavior; vitamin D metabolic process; Schwann cell differentiation; response to genistein; response to hydrogen peroxide; xenobiotic metabolic process; C21-steroid hormone biosynthetic process; sterol metabolic process; granulosa cell differentiation; phenol metabolic process. Disease: Adrenal Insufficiency, Congenital, With 46,xy Sex Reversal, Partial Or Complete

0.05 mL

285 USD

0.2 mL

555