NR0B2 Polyclonal Antibody | MyBioSource MBS127945 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

NR0B2 Polyclonal Antibody

catalog :

MBS127945

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using NR0B2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

image 2 :

Immunohistochemistry of paraffin-embedded rat liver using NR0B2 antibody at dilution of 1:100 (40x lens).

image 3 :

Immunofluorescence analysis of U2OS cells using NR0B2 antibody. Blue: DAPI for nuclear staining.

product information

catalog number :

MBS127945

products type :

Antibody

products full name :

NR0B2 Polyclonal Antibody

products short name :

[NR0B2]

products name syn :

[SHP; SHP1]

other names :

[Nuclear receptor subfamily 0 group B member 2; Nuclear receptor subfamily 0 group B member 2; nuclear receptor subfamily 0 group B member 2; nuclear receptor SHP; small heterodimer partner; orphan nuclear receptor SHP; nuclear receptor subfamily 0, group B, member 2; Orphan nuclear receptor SHP; Small heterodimer partner]

products gene name :

[NR0B2]

other gene names :

[NR0B2; NR0B2; SHP; SHP1; SHP]

uniprot entry name :

NR0B2_HUMAN

clonality :

polyclonal

isotype :

IgG

host :

unknown

reactivity :

Human, Mouse, Rat

sequence length :

257

purity :

Affinity Purification

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:10 - 1:100

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Source: Rabbit

other info2 :

Immunogen: Recombinant protein of human NR0B2. Calculated Molecular Weight: 28kDa. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products categories :

Polyclonal

products description :

The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function.

ncbi gi num :

9978744

ncbi acc num :

Q15466.2

uniprot acc num :

Q15466

ncbi pathways :

Androgen Receptor Signaling Pathway (198806); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Nuclear Receptor Transcription Pathway (105979)

ncbi summary :

uniprot summary :

NR0B2: Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box- containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1. Defects in NR0B2 may be associated with obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the nuclear hormone receptor family. NR0 subfamily. Protein type: Nuclear receptor co-regulator; Nuclear receptor; Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 1p36.1. Cellular Component: nucleoplasm; protein complex; cytoplasm; nucleus. Molecular Function: retinoid X receptor binding; protein domain specific binding; protein binding; peroxisome proliferator activated receptor binding; protein homodimerization activity; DNA binding; protein complex binding; steroid hormone receptor activity; thyroid hormone receptor binding; transcription factor activity; transcription corepressor activity. Biological Process: cholesterol metabolic process; transcription initiation from RNA polymerase II promoter; organ regeneration; negative regulation of transcription factor activity; response to glucose stimulus; positive regulation of insulin secretion; steroid hormone mediated signaling; gene expression; negative regulation of transcription from RNA polymerase II promoter. Disease: Obesity

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage