CDK4 Polyclonal Antibody | MyBioSource MBS127817 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CDK4 Polyclonal Antibody

catalog :

MBS127817

quantity :

0.05 mL

price :

230 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS127817

products type :

Antibody

products full name :

CDK4 Polyclonal Antibody

products short name :

CDK4

products name syn :

CDK4; CMM3; MGC14458; PSK-J3

other names :

Cyclin-dependent kinase 4; Cyclin-dependent kinase 4; cyclin-dependent kinase 4; cell division protein kinase 4; cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3

products gene name :

CDK4

other gene names :

CDK4; CDK4; CMM3; PSK-J3

uniprot entry name :

CDK4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

303

purity :

Affinity Purification

concentration :

1mg/ml

storage stability :

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:20 - 1:100

other info1 :

Species: Human. Immunogen: Recombinant Protein

other info2 :

Immunogen: Center-peptide of human CDK4. Calculated Molecular Weight: 34kDa

products categories :

Polyclonal

products description :

Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase (2). The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle (4).

ncbi gi num :

1168867

ncbi acc num :

P11802.2

uniprot acc num :

P11802

ncbi mol weight :

303

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); B Cell Receptor Signaling Pathway (198909); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463)

ncbi summary :

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008]

uniprot summary :

CDK4: a protein kinase of the CDK family that is important for cell cycle G1 phase progression. Its activity is restricted to the G1-S phase. Controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). Phosphorylates the retinoblastoma gene product (Rb). Point mutations found in somatic and familial melanoma. Amplified in sarcomas, glioma and lymphoma. Amplified, methylated or deleted in head and neck squamous cell carcinoma. Overexpression drives epithelial tumors in mice. Disruption makes mice resistant to cancer. Inhibitor: PD332991. Protein type: Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; EC 2.7.11.22; Kinase, protein; CMGC group; CDK family; CDK4 subfamily; CDK/CDK4 subfamily. Chromosomal Location of Human Ortholog: 12q14. Cellular Component: nucleoplasm; transcription factor complex; nuclear membrane; tight junction; perinuclear region of cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleolus; nucleus; chromatin; cytosol. Molecular Function: cyclin binding; protein binding; cyclin-dependent protein kinase activity; protein complex binding; cyclin-dependent protein kinase regulator activity; ATP binding. Biological Process: response to drug; lens development in camera-type eye; circadian rhythm; establishment and/or maintenance of chromatin architecture; organ regeneration; positive regulation of cell size; positive regulation of translation; positive regulation of apoptosis; response to toxin; response to testosterone stimulus; signal transduction; protein amino acid phosphorylation; positive regulation of fibroblast proliferation; response to hyperoxia; cell division; regulation of gene expression; positive regulation of cell proliferation; response to lead ion; mitotic cell cycle; regulation of protein kinase activity; G1/S transition of mitotic cell cycle. Disease: Melanoma, Cutaneous Malignant, Susceptibility To, 3

size1 :

0.05 mL

price1 :

230 USD

size2 :

0.1 mL

price2 :

305

size3 :

0.2 mL

price3 :

460

more info or order :

MyBioSource product webpage