ADRBK1 Polyclonal Antibody | MyBioSource MBS127677 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ADRBK1 Polyclonal Antibody

catalog :

MBS127677

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using ADRBK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

image 2 :

Immunohistochemistry of paraffin-embedded rat brain using ADRBK1 Antibody at dilution of 1:200 (40x lens).

image 3 :

Immunohistochemistry of paraffin-embedded rat heart using ADRBK1 Antibody at dilution of 1:200 (40x lens).

product information

catalog number :

MBS127677

products type :

Antibody

products full name :

ADRBK1 Polyclonal Antibody

products short name :

[ADRBK1]

products name syn :

[BARK1; BETA-ARK1; FLJ16718; GRK2]

other names :

[Beta-adrenergic receptor kinase 1; Beta-adrenergic receptor kinase 1; beta-adrenergic receptor kinase 1; beta-ARK-1; G-protein coupled receptor kinase 2; adrenergic, beta, receptor kinase 1; G-protein coupled receptor kinase 2]

products gene name :

[ADRBK1]

other gene names :

[ADRBK1; ADRBK1; GRK2; BARK1; BETA-ARK1; BARK; BARK1; GRK2; Beta-ARK-1]

uniprot entry name :

ARBK1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

689

purity :

Affinity Purification

concentration :

1mg/ml

storage stability :

Store at -20 degree C (regular). Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1:500 - 1:2000. IHC: 1:50 - 1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Immunohistochemistry of paraffin-embedded human esophageal cancer using ADRBK1 Antibody at dilution of 1:200 (40x lens).

image5 heading :

Immunohistochemistry (IHC)

image5 description :

Immunohistochemistry of paraffin-embedded mouse kidney using ADRBK1 Antibody at dilution of 1:200 (40x lens).

other info1 :

Species: Human. Immunogen: Recombinant Protein

other info2 :

Immunogen: Recombinant protein of human ADRBK1. Calculated Molecular Weight: 80kDa. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products categories :

Polyclonal

products description :

G-protein-coupled receptor kinase 2 (GRK2), also known as beta-adrenergic receptor kinase 1 (beta-ARK1), is a member of the GRK family, which phosphorylates the activated form of G-protein-coupled receptors (GPCRs) and initiates the desensitization process of GPCR (1). GRK2 kinase activity and cellular localization are tightly regulated by interactions with activated receptors, G-beta and G-gamma subunits, adaptor proteins, phospholipids, caveolin and calmodulin, as well as by phosphorylation (1). PKC phosphorylation enhances GRK2 activity by promoting its membrane localization and by abolishing the inhibitory association of calmodulin (2,3). PKA phosphorylates GRK2 at Ser685, which facilitates the association of GRK2 with a beta-adrenergic receptor (4). Erk inhibits GRK2 activity via phosphorylation at Ser670 (5). Src phosphorylates GRK2 at multiple tyrosine residues (Tyr13, 86 and 92), which activates GRK2 activity and promotes GRK2 degradation (6,7).

ncbi gi num :

126302521

ncbi acc num :

P25098.2

uniprot acc num :

P25098

ncbi pathways :

Activation Of SMO Pathway (1127544); CXCR4-mediated Signaling Events Pathway (137910); Ca-dependent Events Pathway (106497); CaM Pathway (106468); Calmodulin Induced Events Pathway (106469); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); DAG And IP3 Signaling Pathway (477115); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550)

ncbi summary :

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008]

uniprot summary :

GRK2: a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function. Protein type: Kinase, protein; EC 2.7.11.15; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; GRK family; BARK subfamily. Chromosomal Location of Human Ortholog: 11q13.1. Cellular Component: membrane; cytoplasm; plasma membrane; cytosol. Molecular Function: G-protein coupled receptor kinase activity; protein binding; Edg-2 lysophosphatidic acid receptor binding; beta-adrenergic receptor kinase activity; alpha-2A adrenergic receptor binding; ATP binding; protein kinase activity. Biological Process: epidermal growth factor receptor signaling pathway; desensitization of G-protein coupled receptor protein signaling pathway; tachykinin signaling pathway; fibroblast growth factor receptor signaling pathway; acetylcholine receptor signaling, muscarinic pathway; nerve growth factor receptor signaling pathway; heart development; peptidyl-threonine phosphorylation; signal transduction; negative regulation of striated muscle contraction; peptidyl-serine phosphorylation; negative regulation of the force of heart contraction by chemical signal; phospholipase C activation; receptor internalization; positive regulation of catecholamine secretion; innate immune response; cardiac muscle contraction

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage