MCT4 Polyclonal Antibody | MyBioSource MBS127546 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MCT4 Polyclonal Antibody

catalog :

MBS127546

quantity :

0.1 mL

price :

275 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of NCI-H460 cells, using SLC16A3 antibody. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

product information

catalog number :

MBS127546

products type :

Antibody

products full name :

MCT4 Polyclonal Antibody

products short name :

[MCT4]

products name syn :

[SLC16A3; MCT4; Monocarboxylate transporter 4]

other names :

[Monocarboxylate transporter 4; Monocarboxylate transporter 4; monocarboxylate transporter 4; monocarboxylate transporter 3; solute carrier family 16 (monocarboxylic acid transporters), member 3; solute carrier family 16, member 3 (monocarboxylic acid transporter 4); solute carrier family 16 (monocarboxylate transporter), member 3; Solute carrier family 16 member 3]

products gene name :

[SLC16A3]

other gene names :

[SLC16A3; SLC16A3; MCT3; MCT4; MCT 3; MCT 4; MCT-3; MCT-4; MCT4; MCT 4]

uniprot entry name :

MOT4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

465

purity :

Affinity Purification

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1:200 - 1:500. IHC: 1:50 - 1:100

image1 heading :

Western Blot (WB)

other info1 :

Species: Human. Route: Synthetic Peptide. Category: Primary antibody

other info2 :

Immunogen: A synthetic peptide of human MCT4. Calculated Molecular Weight: 49kDa. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products categories :

Polyclonal

products description :

Monocarboxylates, such as lactate and pyruvate, play an integral role in cellular metabolism. Lactic acid is produced in large quantities as a result of glycolysis, which provides the majority of ATP to cells under normal physiological conditions (2). However, accumulation of lactic acid leads to a decrease in intracellular pH and cessation of glycolysis (2). In order for glycolysis to continue at a high rate, lactic acid must be transported out of the cell (1,2). This transport process is carried out by a family of monocarboxylate transporters (MCTs), which function as proton symports and are stereoselective for L-lactate (1 -3).The MCT family consists of at least eight members, MCT 1-8, which contain between 10-12 transmembrane-helical (TM) domains, with the amino and carboxy termini located in the cytoplasm (4). MCT1 is widely expressed and is the major form of MCT in tumor cells and erythrocytes (5,6). MCT2 is highly expressed in liver and testis, while MCT3 and MCT4 are predominantly expressed in skeletal muscle (7,8).

ncbi gi num :

6225705

ncbi acc num :

O15427.1

uniprot acc num :

O15427

ncbi pathways :

Basigin Interactions Pathway (106065); Cell Surface Interactions At The Vascular Wall Pathway (106062); Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); Hemostasis Pathway (106028); Metabolism Pathway (477135); Proton-coupled Monocarboxylate Transport Pathway (161065); Pyruvate Metabolism Pathway (106205); Pyruvate Metabolism And Citric Acid (TCA) Cycle Pathway (106327); SLC-mediated Transmembrane Transport Pathway (119558)

ncbi summary :

Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton-linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family-16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 (SLC16A1; MIM 600682) and MCT2 (SLC16A7; MIM 603654), are characterized by 12 predicted transmembrane domains (Price et al., 1998 [PubMed 9425115]).[supplied by OMIM, Mar 2008]

uniprot summary :

SLC16A3: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. Protein type: Membrane protein, multi-pass; Transporter; Membrane protein, integral; Transporter, SLC family. Chromosomal Location of Human Ortholog: 17q25. Cellular Component: nuclear membrane; membrane; integral to plasma membrane; plasma membrane; actin cytoskeleton. Molecular Function: monocarboxylic acid transmembrane transporter activity; symporter activity; lactate transmembrane transporter activity; secondary active monocarboxylate transmembrane transporter activity. Biological Process: cellular metabolic process; monocarboxylic acid transport; pyruvate metabolic process; blood coagulation; leukocyte migration; transmembrane transport

size1 :

0.1 mL

price1 :

275 USD

size2 :

0.2 mL

price2 :

430

more info or order :

MyBioSource product webpage