product summary
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company name :
MyBioSource
product type :
antibody
product name :
CD33 Polyclonal Antibody
catalog :
MBS127495
quantity :
0.05 mL
price :
200 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
image
image 1 :
Western blot analysis of extracts of various cell lines, using CD33 Antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
image 2 :
Immunohistochemistry of paraffin-embdded human tonsil using CD33 antibody at dilution of 1:100 (40x lens).
image 3 :
Immunofluorescence analysis of THP-1 cells using CD33 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
product information
catalog number :
MBS127495
products type :
Antibody
products full name :
CD33 Polyclonal Antibody
products short name :
[CD33]
products name syn :
[FLJ00391; SIGLEC-3; SIGLEC3; p67]
other names :
[Myeloid cell surface antigen CD33; Myeloid cell surface antigen CD33; myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; CD33 molecule; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD_antigen: CD33]
products gene name :
[CD33]
other gene names :
[CD33; CD33; p67; SIGLEC3; SIGLEC-3; SIGLEC3; Siglec-3]
uniprot entry name :
CD33_HUMAN
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human, Mouse, Rat
sequence length :
364
purity :
Affinity Purification
storage stability :
Store at -20 degree c. Avoid freeze / thaw cycles.
tested application :
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
app notes :
WB: 1:500 - 1:2000. IHC: 1:50 - 1:200
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry
image3 heading :
Immunofluorescence
other info1 :
Species: Human. Immunogen: Recombinant Protein
other info2 :
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 49-259 of human CD33 (NP_001763.3). Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
products categories :
Polyclonal
products description :
CD33 is a type I transmembrane glycoprotein that is found on granulocyte and macrophage precursors in the bone marrow, but is absent from pluripotent stem cells. CD33 is also expressed on monocytes in peripheral blood. It is used as a marker to distinguish myelogenous leukemia cells from lymphoid or erythroid leukemias. CD33 may function as a sialic acid-dependent cell adhesion molecule.
ncbi gi num :
116241290
ncbi acc num :
P20138.2
uniprot acc num :
P20138
ncbi mol weight :
Calculated MW: 25kDa/33kDa/39kDa. Observed MW: 67kDa
ncbi pathways :
Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489)
uniprot summary :
CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Protein type: Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 19q13.3. Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane. Molecular Function: protein binding; receptor activity; carbohydrate binding. Biological Process: negative regulation of cell proliferation; cell-cell signaling; signal transduction; cell adhesion
size1 :
0.05 mL
price1 :
200 USD
size2 :
0.1 mL
price2 :
275
size3 :
0.2 mL
price3 :
430
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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