NDP Polyclonal Antibody | MyBioSource MBS127258 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

NDP Polyclonal Antibody

catalog :

MBS127258

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using NDP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

product information

catalog number :

MBS127258

products type :

Antibody

products full name :

NDP Polyclonal Antibody

products short name :

[NDP]

products name syn :

[ND; EVR2; FEVR]

other names :

[Norrin; Norrin; norrin; norrie disease protein; X-linked exudative vitreoretinopathy 2 protein; Norrie disease (pseudoglioma); Norrie disease protein; X-linked exudative vitreoretinopathy 2 protein]

products gene name :

[NDP]

other gene names :

[NDP; NDP; ND; EVR2; FEVR; EVR2]

uniprot entry name :

NDP_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

133

purity :

Affinity Purification

concentration :

1mg/ml

storage stability :

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1:500 - 1:2000. IHC: 1:50 - 1:200

image1 heading :

Western Blot (WB)

other info1 :

Category: Primary antibody. Species: Human

other info2 :

Immunogen: Recombinant protein of human NDP. Calculated Molecular Weight: 15kDa. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products categories :

Polyclonal

products description :

This gene encodes a secreted protein with a cystein-knot motif that activates the Wnt/beta-catenin pathway. The protein forms disulfide-linked oligomers in the extracellular matrix. Mutations in this gene result in Norrie disease and X-linked exudative vitreoretinopathy.

ncbi gi num :

548342

ncbi acc num :

Q00604.1

uniprot acc num :

Q00604

ncbi summary :

uniprot summary :

NDP: Activates the canonical Wnt signaling pathway through FZD4 and LRP5 coreceptor. Plays a central role in retinal vascularization by acting as a ligand for FZD4 that signals via stabilizing beta-catenin (CTNNB1) and activating LEF/TCF-mediated transcriptional programs. Acts in concert with TSPAN12 to activate FZD4 independently of the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1). May be involved in a pathway that regulates neural cell differentiation and proliferation. Possible role in neuroectodermal cell-cell interaction. Defects in NDP are the cause of Norrie disease (ND); also known as atrophia bulborum hereditaria or Episkopi blindness. ND is a recessive disorder characterized by very early childhood blindness due to degenerative and proliferative changes of the neuroretina. Approximately 50% of patients show some form of progressive mental disorder, often with psychotic features, and about one-third of patients develop sensorineural deafness in the second decade. In addition, some patients have more complex phenotypes, including growth failure and seizure. Defects in NDP are the cause of vitreoretinopathy exudative type 2 (EVR2). EVR2 is a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. This may lead to compensatory retinal neovascularization, which is thought to be induced by hypoxia from the initial avascular insult. New vessels are prone to leakage and rupture causing exudates and bleeding, followed by scarring, retinal detachment and blindness. Clinical features can be highly variable, even within the same family. Patients with mild forms of the disease are asymptomatic, and their only disease related abnormality is an arc of avascular retina in the extreme temporal periphery. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: Xp11.4. Cellular Component: extracellular matrix; extracellular space; cell surface. Molecular Function: protein binding; protein homodimerization activity; growth factor activity; frizzled binding; cytokine activity. Biological Process: nervous system development; cell proliferation; sensory perception of sound; Wnt receptor signaling pathway; visual perception; cell-cell signaling; positive regulation of transcription, DNA-dependent; positive regulation of transcription factor activity; Wnt receptor signaling pathway through beta-catenin; signal transduction; vacuole organization and biogenesis; placenta development. Disease: Exudative Vitreoretinopathy 2, X-linked; Norrie Disease

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage