antibody

BTRC Polyclonal Antibody

MBS127143

0.05 mL

200 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry

Antibody

BTRC Polyclonal Antibody

[BTRC]

[BETA-TRCP; FBW1A; FBXW1; FBXW1A; FWD1; MGC4643; bTrCP; bTrCP1; betaTrCP]

[F-box/WD repeat-containing protein 1A; F-box/WD repeat-containing protein 1A; F-box/WD repeat-containing protein 1A; beta-TrCP1; E3RSIkappaB; F-box and WD-repeat protein 1B; epididymis tissue protein Li 2a; pIkappaBalpha-E3 receptor subunit; F-box and WD repeats protein beta-TrCP; beta-transducin repeat containing E3 ubiquitin protein ligase; E3RSIkappaB; Epididymis tissue protein Li 2a; F-box and WD repeats protein beta-TrCP; pIkappaBalpha-E3 receptor subunit]

[BTRC]

[BTRC; BTRC; FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP; BTRCP; FBW1A; FBXW1A]

FBW1A_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

605

Affinity Purification

1mg/ml

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50-1:200

Western Blot (WB)

Species: Human. Immunogen: Recombinant Protein

Immunogen: Recombinant protein of human BTRC. Calculated Molecular Weight: 69kDa

Polyclonal

beta-transducin repeat-containing protein (beta-TrCP or FBW1A) is an F-box family protein characterized by the presence of the protein-protein mediating F-box domain first described in cyclin F. F-box proteins act as substrate adaptors that target proteins containing a specific phosphorylated sequence element, referred to as a phosphodegron, to the SCF E3 ubiquitin ligase complex for ubiquitination (1,2). beta-TrCP targets many important proteins with diverse functions, such as p53, H-Ras, Smad4, IkappaBalpha, beta-catenin, and the cell cycle checkpoint protein claspin, for ubiquitin-mediated degradation (3-5). Research studies have shown that inhibition of beta-TrCP expression has a demonstrated benefit in the treatment of prostate cancer (6).

13124271

Q9Y297.1

Q9Y297

605

AMER1 Mutants Destabilize The Destruction Complex Pathway (1127621); APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway (1127616); APC Truncation Mutants Have Impaired AXIN Binding Pathway (1127617); APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (105825); AXIN Missense Mutants Destabilize The Destruction Complex Pathway (1127620); AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway (1127618); Activation Of NF-kappaB In B Cells Pathway (576251); Adaptive Immune System Pathway (366160); Alternative NF-kappaB Pathway (138004); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162)

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar 2012]

BTRC: a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Regulates the stability of CTNNB1 and participates in Wnt signaling. Interacts directly with SKP1 in the SCF complex. Interacts specifically with phosphorylated CTNNB1 and NFKBIA, ubiquitination substrates. Two alternatively spliced isoforms have been described. Protein type: Ubiquitin conjugating system; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 10q24.32. Cellular Component: nucleoplasm; SCF ubiquitin ligase complex; cytosol. Molecular Function: protein dimerization activity; protein phosphorylated amino acid binding; protein binding; ubiquitin-protein ligase activity; beta-catenin binding; ligase activity. Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; circadian rhythm; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; viral reproduction; regulation of cell cycle; positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; protein ubiquitination; positive regulation of circadian rhythm; protein amino acid dephosphorylation; signal transduction; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; G2/M transition of mitotic cell cycle; regulation of I-kappaB kinase/NF-kappaB cascade; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway; protein destabilization; negative regulation of transcription factor activity; mammary gland epithelial cell proliferation; regulation of circadian rhythm; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of smoothened signaling pathway; mitotic cell cycle; negative regulation of transcription, DNA-dependent

0.05 mL

200 USD

0.1 mL

275

0.2 mL

430